Aphanizomenon flos-Aquae (AFA) cyanobacteria from Klamath Lake (Oregon) are considered a "superfood" due to their broad nutritional profile that has proved to have health-enhancing properties. The AFA metabolome is quite complex. Here, we present a study that, combining multinuclear 1H, 31P, and 13C Nuclear Magnetic Resonance (NMR) spectroscopy and high-resolution mass spectrometry, led to the detection of uncommon phosphorylated metabolites in AFA. We focused our attention on 31P NMR signals at 20 ppm, a chemical shift that usually points to the presence of phosphonates. The molecules contributing to 20 ppm 31P NMR signals revealed, instead, to be nucleoside 2′,3′-cyclic monophosphates. These metabolites were fully characterized by multinuclear 1H, 31P, and 13C NMR spectroscopy and high-resolution mass spectrometry.
Zambon A., Righi V., Parenti F., Libertini E., Rossi M.C., Mucci A. (2019). Nucleoside 2′,3′-Cyclic Monophosphates in Aphanizomenon flos-Aquae Detected through Nuclear Magnetic Resonance and Mass Spectrometry. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 67(46), 12780-12785 [10.1021/acs.jafc.9b05991].
Nucleoside 2′,3′-Cyclic Monophosphates in Aphanizomenon flos-Aquae Detected through Nuclear Magnetic Resonance and Mass Spectrometry
Righi V.;
2019
Abstract
Aphanizomenon flos-Aquae (AFA) cyanobacteria from Klamath Lake (Oregon) are considered a "superfood" due to their broad nutritional profile that has proved to have health-enhancing properties. The AFA metabolome is quite complex. Here, we present a study that, combining multinuclear 1H, 31P, and 13C Nuclear Magnetic Resonance (NMR) spectroscopy and high-resolution mass spectrometry, led to the detection of uncommon phosphorylated metabolites in AFA. We focused our attention on 31P NMR signals at 20 ppm, a chemical shift that usually points to the presence of phosphonates. The molecules contributing to 20 ppm 31P NMR signals revealed, instead, to be nucleoside 2′,3′-cyclic monophosphates. These metabolites were fully characterized by multinuclear 1H, 31P, and 13C NMR spectroscopy and high-resolution mass spectrometry.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
