Direct investigation of the early cellular changes induced by metastatic cells within the surrounding tissue remains a challenge. Here we present a system in which metastatic cancer cells release a cell-penetrating fluorescent protein, which is taken up by neighbouring cells and enables spatial identification of the local metastatic cellular environment. Using this system, tissue cells with low representation in the metastatic niche can be identified and characterized within the bulk tissue. To highlight its potential, we applied this strategy to study the cellular environment of metastatic breast cancer cells in the lung. We report the presence of cancer-associated parenchymal cells, which exhibit stem-cell-like features, expression of lung progenitor markers, multi-lineage differentiation potential and self-renewal activity. In ex vivo assays, lung epithelial cells acquire a cancer-associated parenchymal-cell-like phenotype when co-cultured with cancer cells and support their growth. These results highlight the potential of this method as a platform for new discoveries.

Ombrato L., Nolan E., Kurelac I., Mavousian A., Bridgeman V.L., Heinze I., et al. (2019). Metastatic-niche labelling reveals parenchymal cells with stem features. NATURE, 572(7771), 603-608 [10.1038/s41586-019-1487-6].

Metastatic-niche labelling reveals parenchymal cells with stem features

Kurelac I.;WESTON, ANITA;ORI, ANDREA;
2019

Abstract

Direct investigation of the early cellular changes induced by metastatic cells within the surrounding tissue remains a challenge. Here we present a system in which metastatic cancer cells release a cell-penetrating fluorescent protein, which is taken up by neighbouring cells and enables spatial identification of the local metastatic cellular environment. Using this system, tissue cells with low representation in the metastatic niche can be identified and characterized within the bulk tissue. To highlight its potential, we applied this strategy to study the cellular environment of metastatic breast cancer cells in the lung. We report the presence of cancer-associated parenchymal cells, which exhibit stem-cell-like features, expression of lung progenitor markers, multi-lineage differentiation potential and self-renewal activity. In ex vivo assays, lung epithelial cells acquire a cancer-associated parenchymal-cell-like phenotype when co-cultured with cancer cells and support their growth. These results highlight the potential of this method as a platform for new discoveries.
2019
Ombrato L., Nolan E., Kurelac I., Mavousian A., Bridgeman V.L., Heinze I., et al. (2019). Metastatic-niche labelling reveals parenchymal cells with stem features. NATURE, 572(7771), 603-608 [10.1038/s41586-019-1487-6].
Ombrato L.; Nolan E.; Kurelac I.; Mavousian A.; Bridgeman V.L.; Heinze I.; Chakravarty P.; Horswell S.; Gonzalez-Gualda E.; Matacchione G.; Weston A.;...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/707073
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