According to previous research, natural polyamines exert a role in regulating cell committment and differentiation from stemness during skeletal development. In order to assess whether distinct polyamine patterns are associated with different skeletal cell types, primary cultures of stem cells, chondrocytes or osteoblasts were dedicated for HPLC analysis of intracellular polyamines. Spermine (SPM) and Spermidine (SPD) levels were higher in adipose derived stem cells (ASC) compared to mature skeletal cells, i.e. chondrocytes and osteoblasts, confirming the connection of polyamine content with stemness. To establish whether polyamines can protect ASC against oxidative DNA damage in a 3-D differentiation model, the level of gamma H2AX was measured by western blot, and found to correlate with age and BMI of patients. Addition of either polyamine to ASC was able to hinder DNA damage in the low micromolecular range, with marked reduction of gamma H2AX level at 10 mu M SPM and 5 mu M SPD. Molecular analysis of the mechanisms that might underlie the protective effect of polyamine supplementation evidences a possible involvement of autophagy. Altogether, these results support the idea that polyamines are able to manage both stem cell differentiation and cell oxidative damage, and therefore represent appealing tools for regenerative and cell based applications.

Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D / Minguzzi M.; Guidotti S.; Platano D.; D'Adamo S.; Cetrullo S.; Assirelli E.; Santi S.; Mariani E.; Trisolino G.; Filardo G.; Flamigni F.; Borzi R.M.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - ELETTRONICO. - 9:1(2019), pp. 14269.1-14269.13. [10.1038/s41598-019-50543-z]

Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D

Minguzzi M.;Guidotti S.;D'Adamo S.;Cetrullo S.;Mariani E.;Flamigni F.;
2019

Abstract

According to previous research, natural polyamines exert a role in regulating cell committment and differentiation from stemness during skeletal development. In order to assess whether distinct polyamine patterns are associated with different skeletal cell types, primary cultures of stem cells, chondrocytes or osteoblasts were dedicated for HPLC analysis of intracellular polyamines. Spermine (SPM) and Spermidine (SPD) levels were higher in adipose derived stem cells (ASC) compared to mature skeletal cells, i.e. chondrocytes and osteoblasts, confirming the connection of polyamine content with stemness. To establish whether polyamines can protect ASC against oxidative DNA damage in a 3-D differentiation model, the level of gamma H2AX was measured by western blot, and found to correlate with age and BMI of patients. Addition of either polyamine to ASC was able to hinder DNA damage in the low micromolecular range, with marked reduction of gamma H2AX level at 10 mu M SPM and 5 mu M SPD. Molecular analysis of the mechanisms that might underlie the protective effect of polyamine supplementation evidences a possible involvement of autophagy. Altogether, these results support the idea that polyamines are able to manage both stem cell differentiation and cell oxidative damage, and therefore represent appealing tools for regenerative and cell based applications.
2019
Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D / Minguzzi M.; Guidotti S.; Platano D.; D'Adamo S.; Cetrullo S.; Assirelli E.; Santi S.; Mariani E.; Trisolino G.; Filardo G.; Flamigni F.; Borzi R.M.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - ELETTRONICO. - 9:1(2019), pp. 14269.1-14269.13. [10.1038/s41598-019-50543-z]
Minguzzi M.; Guidotti S.; Platano D.; D'Adamo S.; Cetrullo S.; Assirelli E.; Santi S.; Mariani E.; Trisolino G.; Filardo G.; Flamigni F.; Borzi R.M.
File in questo prodotto:
File Dimensione Formato  
SR s41598-019-50543-z.pdf

accesso aperto

Tipo: Versione (PDF) editoriale
Licenza: Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY)
Dimensione 1.8 MB
Formato Adobe PDF
1.8 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/703147
Citazioni
  • ???jsp.display-item.citation.pmc??? 8
  • Scopus 8
  • ???jsp.display-item.citation.isi??? 9
social impact