A fluorescent derivative of trehalose with two dansyl groups (DAT) has been synthesized. It is characterised by a large Stokes shift, good permeability in human living cells and a well detectable fluorescent signal within the cells. Notably, in intestinal cells DAT is sequestered in vesicles induced by trehalose pretreatment and colocalizes with lipid droplets.
Dansyl acetyl trehalose: a novel tool to investigate the cellular fate of trehalose / Alessandra Locatelli, Luisa Iommarini, Alessandra Graziadio, Alberto Leoni, Anna Maria Porcelli, Stefano Iotti, Emil Malucelli, Francesco Francia, Giovanni Venturoli, Giovanna Farruggia. - In: RSC ADVANCES. - ISSN 2046-2069. - ELETTRONICO. - 9:27(2019), pp. 15350-15356. [10.1039/c9ra01800j]
Dansyl acetyl trehalose: a novel tool to investigate the cellular fate of trehalose
Alessandra Locatelli;Luisa Iommarini;Alessandra Graziadio;Alberto Leoni;Anna Maria Porcelli;Stefano Iotti;Emil Malucelli;Francesco Francia;Giovanni Venturoli;Giovanna Farruggia
2019
Abstract
A fluorescent derivative of trehalose with two dansyl groups (DAT) has been synthesized. It is characterised by a large Stokes shift, good permeability in human living cells and a well detectable fluorescent signal within the cells. Notably, in intestinal cells DAT is sequestered in vesicles induced by trehalose pretreatment and colocalizes with lipid droplets.| File | Dimensione | Formato | |
|---|---|---|---|
|
Dansyl acetyl trehalose a novel tool to investigate RCS advances 2019.pdf
accesso aperto
Tipo:
Versione (PDF) editoriale
Licenza:
Licenza per Accesso Aperto. Creative Commons Attribuzione - Non commerciale (CCBYNC)
Dimensione
1.08 MB
Formato
Adobe PDF
|
1.08 MB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
