A fluorescent derivative of trehalose with two dansyl groups (DAT) has been synthesized. It is characterised by a large Stokes shift, good permeability in human living cells and a well detectable fluorescent signal within the cells. Notably, in intestinal cells DAT is sequestered in vesicles induced by trehalose pretreatment and colocalizes with lipid droplets.
Dansyl acetyl trehalose: a novel tool to investigate the cellular fate of trehalose
Alessandra Locatelli;Luisa Iommarini;Alessandra Graziadio;Alberto Leoni;Anna Maria Porcelli;Stefano Iotti;Emil Malucelli;Francesco Francia;Giovanni Venturoli;Giovanna Farruggia
2019
Abstract
A fluorescent derivative of trehalose with two dansyl groups (DAT) has been synthesized. It is characterised by a large Stokes shift, good permeability in human living cells and a well detectable fluorescent signal within the cells. Notably, in intestinal cells DAT is sequestered in vesicles induced by trehalose pretreatment and colocalizes with lipid droplets.File in questo prodotto:
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Dansyl acetyl trehalose a novel tool to investigate RCS advances 2019.pdf
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