Aim: HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens. Materials & methods: We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the presence of 16 HPV DNA types. Results: We show that the presence of HPV DNA in the ND is predictive of HPV DNA-positive breast lesions and that HPV DNA is more represented in intraductal papillomas.We also show the presence of HPV DNA in the serum-derived extracellular vesicles. Conclusion: Our data supports the use of liquid biopsy to detect HPV DNA in breast pathology
De Carolis, S., Pellegrini, A., Santini, D., Ceccarelli, C., De Leo, A., Alessandrini, F., et al. (2018). Liquid biopsy in the diagnosis of HPV DNA in breast lesions. FUTURE MICROBIOLOGY, 13(2), 187-194 [10.2217/fmb-2017-0145].
Liquid biopsy in the diagnosis of HPV DNA in breast lesions
De Carolis, Sabrina;PELLEGRINI, ALICE;Santini, Donatella;Ceccarelli, Claudio;De Leo, Antonio;Alessandrini, Federica;Tesei, Anna;Mantovani, Vilma;Zamagni, Claudio;Taffurelli, Mario;Sansone, Pasquale;Bonafé, Massimiliano;Cricca, Monica
2018
Abstract
Aim: HPV DNA has never been investigated in nipple discharges (ND) and serum-derived extracellular vesicles, although its presence has been reported in ductal lavage fluids and blood specimens. Materials & methods: We analyzed 50 ND, 22 serum-derived extracellular vesicles as well as 51 pathologic breast tissues for the presence of 16 HPV DNA types. Results: We show that the presence of HPV DNA in the ND is predictive of HPV DNA-positive breast lesions and that HPV DNA is more represented in intraductal papillomas.We also show the presence of HPV DNA in the serum-derived extracellular vesicles. Conclusion: Our data supports the use of liquid biopsy to detect HPV DNA in breast pathologyI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
