Differentiation of modern and ancient varieties of common wheat byquantitative capillary electrophoretic profile of phenolic acids

Phenolic compounds have received great attention among the health promoting phytochemicals in com-mon wheat (Triticum aestivum L.), mainly because of their strong antioxidant properties. In the presentstudy a simple Capillary Zone Electrophoresis (CZE) method with UV detection was optimized and vali-dated for the quantitation of six of the most important phenolic acids in whole grain i.e., sinapic, ferulic,syringic, p-coumaric, vanillic and p-hydroxybenzoic acid. The separation was achieved in a running buffercomposed of sodium phosphate solution (50 mM) in water/methanol 80:20 (v/v) at pH 6.0 and using afused-silica capillary at the temperature of 30◦C under application of 27 kV. By means of diode arraydetector, and made possible by the favorable characteristic UV spectra, the quantitation of the soluteswas carried out at 200, 220 and 300 nm, in the complex matrices represented by the soluble and boundfractions of wheat flours. The validation parameters of the method i.e., linearity, sensitivity, precision,accuracy and robustness were in line with those obtained by consolidated separation techniques appliedfor the same purposes (e.g., HPLC-UV), with a significant advantage in term of analysis time (less than12 min). Ten varieties of soft wheat (five modern Italian and five old Italian genotypes) were analysed andthe data were subjected to Principal Components Analysis (PCA). Interestingly, significant differences ofthe quantitative phenolic acids profile were observed between the modern and the ancient genotypes,with the latter showing higher amount of the main represented phenolic acids.