The effect of an atmospheric pressure non-equilibrium plasma on human mesenchymal stem cells was investigated. A dielectric barrier discharge non-equilibrium plasma source driven by two different high-voltage pulsed generators was used and cell survival, senescence, proliferation, and differentiation were evaluated. Cells deprived of the culture medium and treated with nanosecond pulsed plasma showed a higher mortality rate, while higher survival and retention of proliferation were observed in cells treated with microsecond pulsed plasma in the presence of the culture medium. While a few treated cells showed the hallmarks of senescence, unexpected delayed apoptosis ensued in cells exposed to plasma-treated medium. The plasma treatment did not change the expression of OCT4, a marker of mesenchymal stem cell differentiation.

R Laurita, F Alviano, C Marchionni, P M Abruzzo, A Bolotta, L Bonsi, et al. (2016). A study of the effect on human mesenchymal stem cells of an atmospheric pressure plasma source driven by different voltage waveforms. JOURNAL OF PHYSICS. D, APPLIED PHYSICS, 49(36), 1-14 [10.1088/0022-3727/49/36/364003].

A study of the effect on human mesenchymal stem cells of an atmospheric pressure plasma source driven by different voltage waveforms

LAURITA, ROMOLO;ALVIANO, FRANCESCO;MARCHIONNI, COSETTA;ABRUZZO, PROVVIDENZA MARIA;BOLOTTA, ALESSANDRA;BONSI, LAURA;COLOMBO, VITTORIO;GHERARDI, MATTEO;LIGUORI, ANNA;ROSSI, MARTINA;STANCAMPIANO, AUGUSTO;MARINI, MARINA
2016

Abstract

The effect of an atmospheric pressure non-equilibrium plasma on human mesenchymal stem cells was investigated. A dielectric barrier discharge non-equilibrium plasma source driven by two different high-voltage pulsed generators was used and cell survival, senescence, proliferation, and differentiation were evaluated. Cells deprived of the culture medium and treated with nanosecond pulsed plasma showed a higher mortality rate, while higher survival and retention of proliferation were observed in cells treated with microsecond pulsed plasma in the presence of the culture medium. While a few treated cells showed the hallmarks of senescence, unexpected delayed apoptosis ensued in cells exposed to plasma-treated medium. The plasma treatment did not change the expression of OCT4, a marker of mesenchymal stem cell differentiation.
2016
R Laurita, F Alviano, C Marchionni, P M Abruzzo, A Bolotta, L Bonsi, et al. (2016). A study of the effect on human mesenchymal stem cells of an atmospheric pressure plasma source driven by different voltage waveforms. JOURNAL OF PHYSICS. D, APPLIED PHYSICS, 49(36), 1-14 [10.1088/0022-3727/49/36/364003].
R Laurita; F Alviano; C Marchionni; P M Abruzzo; A Bolotta; L Bonsi; V Colombo; M Gherardi; A Liguori; F Ricci; M Rossi; A Stancampiano; P L Tazzari; ...espandi
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/567599
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 5
social impact