The effect of an atmospheric pressure non-equilibrium plasma on human mesenchymal stem cells was investigated. A dielectric barrier discharge non-equilibrium plasma source driven by two different high-voltage pulsed generators was used and cell survival, senescence, proliferation, and differentiation were evaluated. Cells deprived of the culture medium and treated with nanosecond pulsed plasma showed a higher mortality rate, while higher survival and retention of proliferation were observed in cells treated with microsecond pulsed plasma in the presence of the culture medium. While a few treated cells showed the hallmarks of senescence, unexpected delayed apoptosis ensued in cells exposed to plasma-treated medium. The plasma treatment did not change the expression of OCT4, a marker of mesenchymal stem cell differentiation.
A study of the effect on human mesenchymal stem cells of an atmospheric pressure plasma source driven by different voltage waveforms / R Laurita; F Alviano; C Marchionni; P M Abruzzo; A Bolotta; L Bonsi; V Colombo; M Gherardi; A Liguori; F Ricci; M Rossi; A Stancampiano; P L Tazzari; M Marini. - In: JOURNAL OF PHYSICS. D, APPLIED PHYSICS. - ISSN 1361-6463. - ELETTRONICO. - 49:36(2016), pp. 364003.1-364003.14. [10.1088/0022-3727/49/36/364003]
A study of the effect on human mesenchymal stem cells of an atmospheric pressure plasma source driven by different voltage waveforms
LAURITA, ROMOLO;ALVIANO, FRANCESCO;MARCHIONNI, COSETTA;ABRUZZO, PROVVIDENZA MARIA;BOLOTTA, ALESSANDRA;BONSI, LAURA;COLOMBO, VITTORIO;GHERARDI, MATTEO;LIGUORI, ANNA;ROSSI, MARTINA;STANCAMPIANO, AUGUSTO;MARINI, MARINA
2016
Abstract
The effect of an atmospheric pressure non-equilibrium plasma on human mesenchymal stem cells was investigated. A dielectric barrier discharge non-equilibrium plasma source driven by two different high-voltage pulsed generators was used and cell survival, senescence, proliferation, and differentiation were evaluated. Cells deprived of the culture medium and treated with nanosecond pulsed plasma showed a higher mortality rate, while higher survival and retention of proliferation were observed in cells treated with microsecond pulsed plasma in the presence of the culture medium. While a few treated cells showed the hallmarks of senescence, unexpected delayed apoptosis ensued in cells exposed to plasma-treated medium. The plasma treatment did not change the expression of OCT4, a marker of mesenchymal stem cell differentiation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.