Three isolates (A19T, C21 and F12 ) with spiral shaped cells and one bipolar sheathed flagellum were obtained from gastric mucosa and caecal contents of three different wild boars (Sus scrofa) and subjected to a polyphasic taxonomic study. A genus-specific PCR showed that these isolates belonged to the genus Helicobacter. Phylogenetic analysis based on the 16S rRNA, 60 kDa heat-shock protein (HSP60) and atpA genes demonstrated they formed a novel lineage within this genus. Pairwise 16S rRNA, HSP60 and atpA gene sequence comparisons of the three isolates revealed 99.7%, 99.4% and 99.9% sequence identity, respectively, among the three isolates; the 16S rRNA gene of the isolate A19T revealed 98.5% sequence similarity with its nearest validly named neighbor species Helicobacter mastomyrinus (98.5% sequence similarity to the type strain MIT 97-5577T ). The taxonomic uniqueness of the wild boar isolates was confirmed by protein analysis performed by MALDI-TOF mass spectrometry and by a distinctive biochemical profile. These data indicate that the isolates A19T, C21 and F12 represent a novel taxon for which the name Helicobacter apri sp. nov. is proposed, with isolate A19T (= DSM 28990T; LMG 28471T) as the type strain.
Zanoni, R.G., Piva, S., Florio, D., Bassi, P., Mion, D., Cnockaert, M., et al. (2016). Helicobacter apri sp. nov., isolated from wild boars. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 66, 2876-2882 [10.1099/ijsem.0.001071].
Helicobacter apri sp. nov., isolated from wild boars
ZANONI, RENATO GIULIO;PIVA, SILVIA;FLORIO, DANIELA;BASSI, PATRIZIA;MION, DOMENICO;LUCHETTI, ANDREA;
2016
Abstract
Three isolates (A19T, C21 and F12 ) with spiral shaped cells and one bipolar sheathed flagellum were obtained from gastric mucosa and caecal contents of three different wild boars (Sus scrofa) and subjected to a polyphasic taxonomic study. A genus-specific PCR showed that these isolates belonged to the genus Helicobacter. Phylogenetic analysis based on the 16S rRNA, 60 kDa heat-shock protein (HSP60) and atpA genes demonstrated they formed a novel lineage within this genus. Pairwise 16S rRNA, HSP60 and atpA gene sequence comparisons of the three isolates revealed 99.7%, 99.4% and 99.9% sequence identity, respectively, among the three isolates; the 16S rRNA gene of the isolate A19T revealed 98.5% sequence similarity with its nearest validly named neighbor species Helicobacter mastomyrinus (98.5% sequence similarity to the type strain MIT 97-5577T ). The taxonomic uniqueness of the wild boar isolates was confirmed by protein analysis performed by MALDI-TOF mass spectrometry and by a distinctive biochemical profile. These data indicate that the isolates A19T, C21 and F12 represent a novel taxon for which the name Helicobacter apri sp. nov. is proposed, with isolate A19T (= DSM 28990T; LMG 28471T) as the type strain.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
