Three isolates (A19T, C21 and F12 ) with spiral shaped cells and one bipolar sheathed flagellum were obtained from gastric mucosa and caecal contents of three different wild boars (Sus scrofa) and subjected to a polyphasic taxonomic study. A genus-specific PCR showed that these isolates belonged to the genus Helicobacter. Phylogenetic analysis based on the 16S rRNA, 60 kDa heat-shock protein (HSP60) and atpA genes demonstrated they formed a novel lineage within this genus. Pairwise 16S rRNA, HSP60 and atpA gene sequence comparisons of the three isolates revealed 99.7%, 99.4% and 99.9% sequence identity, respectively, among the three isolates; the 16S rRNA gene of the isolate A19T revealed 98.5% sequence similarity with its nearest validly named neighbor species Helicobacter mastomyrinus (98.5% sequence similarity to the type strain MIT 97-5577T ). The taxonomic uniqueness of the wild boar isolates was confirmed by protein analysis performed by MALDI-TOF mass spectrometry and by a distinctive biochemical profile. These data indicate that the isolates A19T, C21 and F12 represent a novel taxon for which the name Helicobacter apri sp. nov. is proposed, with isolate A19T (= DSM 28990T; LMG 28471T) as the type strain.

Helicobacter apri sp. nov., isolated from wild boars

ZANONI, RENATO GIULIO;PIVA, SILVIA;FLORIO, DANIELA;BASSI, PATRIZIA;MION, DOMENICO;LUCHETTI, ANDREA;
2016

Abstract

Three isolates (A19T, C21 and F12 ) with spiral shaped cells and one bipolar sheathed flagellum were obtained from gastric mucosa and caecal contents of three different wild boars (Sus scrofa) and subjected to a polyphasic taxonomic study. A genus-specific PCR showed that these isolates belonged to the genus Helicobacter. Phylogenetic analysis based on the 16S rRNA, 60 kDa heat-shock protein (HSP60) and atpA genes demonstrated they formed a novel lineage within this genus. Pairwise 16S rRNA, HSP60 and atpA gene sequence comparisons of the three isolates revealed 99.7%, 99.4% and 99.9% sequence identity, respectively, among the three isolates; the 16S rRNA gene of the isolate A19T revealed 98.5% sequence similarity with its nearest validly named neighbor species Helicobacter mastomyrinus (98.5% sequence similarity to the type strain MIT 97-5577T ). The taxonomic uniqueness of the wild boar isolates was confirmed by protein analysis performed by MALDI-TOF mass spectrometry and by a distinctive biochemical profile. These data indicate that the isolates A19T, C21 and F12 represent a novel taxon for which the name Helicobacter apri sp. nov. is proposed, with isolate A19T (= DSM 28990T; LMG 28471T) as the type strain.
Zanoni, Renato Giulio; Piva, Silvia; Florio, Daniela; Bassi, Patrizia; Mion, Domenico; Cnockaert, Margo; Luchetti, Andrea; Vandamme, Peter
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/566816
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