Next generation sequencing (NGS) technology has been rapidly introduced into basic and translational research in oncology, but the reduced availability of fresh frozen (FF) tumor tissues and the poor quality of DNA extracted from formalin-fixed, paraffin-embedded (FFPE) has significantly impaired this process in the field of solid tumors. To evaluate if data generated from FFPE material can be reliably produced and potentially used in routine clinical settings, we performed whole exome sequencing (WES) from tumor samples of Gastrointestinal stromal tumors (GIST), either extracted FF or FFPE, and from matched normal DNA.

Whole exome sequencing (WES) on formalin-fixed, paraffin-embedded (FFPE) tumor tissue in gastrointestinal stromal tumors (GIST)

ASTOLFI, ANNALISA;URBINI, MILENA;INDIO, VALENTINA;NANNINI, MARGHERITA;GENOVESE, CHIARA GIUSY;SANTINI, DONATELLA;SAPONARA, MARISTELLA;MANDRIOLI, ANNA;ERCOLANI, GIORGIO;BRANDI, GIOVANNI;BIASCO, GUIDO;PANTALEO, MARIA ABBONDANZA
2015

Abstract

Next generation sequencing (NGS) technology has been rapidly introduced into basic and translational research in oncology, but the reduced availability of fresh frozen (FF) tumor tissues and the poor quality of DNA extracted from formalin-fixed, paraffin-embedded (FFPE) has significantly impaired this process in the field of solid tumors. To evaluate if data generated from FFPE material can be reliably produced and potentially used in routine clinical settings, we performed whole exome sequencing (WES) from tumor samples of Gastrointestinal stromal tumors (GIST), either extracted FF or FFPE, and from matched normal DNA.
Astolfi, Annalisa; Urbini, Milena; Indio, Valentina; Nannini, Margherita; Genovese, Chiara Giusy; Santini, Donatella; Saponara, Maristella; Mandrioli, Anna; Ercolani, Giorgio; Brandi, Giovanni; Biasco, Guido; Pantaleo, Maria A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/526974
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