Molecular detection of monoclonality can play an important role in the diagnosis of posttransplantation lymphoproliferative disorders (PTLD). To permit accurate molecular diagnosis of PTLD even on very small amounts of DNA extracted from routinely embedded histologic material, we adapted a commercially available PCR protocol (for FR-1, -2 and -3 regions), originally designed for use on fresh/frozen samples. We applied this approach on routine biopsy/surgical material of 10 PTLD (from nine patients). All three FR regions were always amplified, indicating that the extracted DNA was of medium quality. All five, PTLD morphologically classified as lymphomas were monoclonal in at least one FR region, Thus, using the WHO histologic, immunohistochemical, and clinical criteria as the reference standard, the approach provided 100 % sensitivity for detection of monoclonal malignancies, supporting the validity of the method. Of five specimens classified morphologically as polymorphic PTLD, three displayed a solitary IgH gene rearrangement peak, consistent with the presence of a monoclonal B-cell population (ie, monoclonal polymorphic PTLD). This rapid and straightforward procedure, which allows identification of a wide range of IgH rear-rangements, could facilitate molecular analysis of PTLD in routine practice, while limiting consumption of valuable diagnostic material,

Application of a fluorescent PCR method for molecular diagnosis of posttransplant lymphoproliferative disorders on routine tissue sections.

PANZA, EMANUELE;GRAZI, GIAN LUCA;PINNA, ANTONIO DANIELE;FIORENTINO, MICHELANGELO;GRIGIONI, FRANCO;D'ERRICO, ANTONIETTA
2005

Abstract

Molecular detection of monoclonality can play an important role in the diagnosis of posttransplantation lymphoproliferative disorders (PTLD). To permit accurate molecular diagnosis of PTLD even on very small amounts of DNA extracted from routinely embedded histologic material, we adapted a commercially available PCR protocol (for FR-1, -2 and -3 regions), originally designed for use on fresh/frozen samples. We applied this approach on routine biopsy/surgical material of 10 PTLD (from nine patients). All three FR regions were always amplified, indicating that the extracted DNA was of medium quality. All five, PTLD morphologically classified as lymphomas were monoclonal in at least one FR region, Thus, using the WHO histologic, immunohistochemical, and clinical criteria as the reference standard, the approach provided 100 % sensitivity for detection of monoclonal malignancies, supporting the validity of the method. Of five specimens classified morphologically as polymorphic PTLD, three displayed a solitary IgH gene rearrangement peak, consistent with the presence of a monoclonal B-cell population (ie, monoclonal polymorphic PTLD). This rapid and straightforward procedure, which allows identification of a wide range of IgH rear-rangements, could facilitate molecular analysis of PTLD in routine practice, while limiting consumption of valuable diagnostic material,
Gruppioni, E; Corti, B; Altimari, A; Gabusi, E; Panza, E; Grazi, GL; Pinna, AD; De Ruvo, N; Fiorentino, M; Grigioni, WF; D'Errico-Grigioni, A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/4948
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