My research project is developed in the framework of the project ICCAT‐GBYP 06/2011 (Atlantic-wide Bluefin Tuna Research Program), in collaboration with several Italian and foreign partners. The study is focused on Thunnus thynnus, a fish species that underwent severe over-exploitation in recent decades. It’s therefore necessary to improve the knowledge about this species and to develop new tool for a better management of the stock. We produced two “omic” resources, the transcriptome (RNA), representative of environmental adaptation and selection, and the genome (DNA), used to create a reference backbone for the analyses of the transcriptome. We used Next Generation Sequencing (NGS) technologies to sequence cDNA libraries from 10 individuals and a genomic reference from 4 individuals. Single Nucleotide Polymorphism (SNP) markers were developed from the sequencing of expressed genes. This class of molecular markers show high statistical power and high frequency in the genome, thus is currently employed for analysis of genetic structure and traceability. In this way we aim to study adaptation of populations to changing environmental conditions, to fishing pressure, to pollution and to other anthropogenic disturbances. By mapping cDNA reads to the genomic reference, we obtained a list of candidate SNPs that was subsequently mined to select a set of 384 loci to be included in the genotyping panel. We will genotype 960 individuals belonging to different size classes and originating from different geographical locations. All sample were collected during 2011. With this dataset we aim to perform population assignment’s studies in order to assign each individual to its population of origin, to estimate degree of admixture between individuals of West and East Mediterranean sea, and to characterize the population structure to evaluate the presence and degree of differentiation of sub-populations of Atlantic Bluefin Tuna.

Genetic structure, demographic trend and traceability of Atlantic and Mediterranean populations of Thunnus thynnus / Pintus E.; Cariani A.; Tinti F.; GBYP-PHASE 2 Consortium. - STAMPA. - (2012), pp. 74-74. (Intervento presentato al convegno VIII Incontro Dottorandi in Ecologia e Scienze dei Sistemi Acquatici tenutosi a Roma, Italia nel 27 February – 1 March 2012).

Genetic structure, demographic trend and traceability of Atlantic and Mediterranean populations of Thunnus thynnus

PINTUS, ELEONORA;CARIANI, ALESSIA;TINTI, FAUSTO;
2012

Abstract

My research project is developed in the framework of the project ICCAT‐GBYP 06/2011 (Atlantic-wide Bluefin Tuna Research Program), in collaboration with several Italian and foreign partners. The study is focused on Thunnus thynnus, a fish species that underwent severe over-exploitation in recent decades. It’s therefore necessary to improve the knowledge about this species and to develop new tool for a better management of the stock. We produced two “omic” resources, the transcriptome (RNA), representative of environmental adaptation and selection, and the genome (DNA), used to create a reference backbone for the analyses of the transcriptome. We used Next Generation Sequencing (NGS) technologies to sequence cDNA libraries from 10 individuals and a genomic reference from 4 individuals. Single Nucleotide Polymorphism (SNP) markers were developed from the sequencing of expressed genes. This class of molecular markers show high statistical power and high frequency in the genome, thus is currently employed for analysis of genetic structure and traceability. In this way we aim to study adaptation of populations to changing environmental conditions, to fishing pressure, to pollution and to other anthropogenic disturbances. By mapping cDNA reads to the genomic reference, we obtained a list of candidate SNPs that was subsequently mined to select a set of 384 loci to be included in the genotyping panel. We will genotype 960 individuals belonging to different size classes and originating from different geographical locations. All sample were collected during 2011. With this dataset we aim to perform population assignment’s studies in order to assign each individual to its population of origin, to estimate degree of admixture between individuals of West and East Mediterranean sea, and to characterize the population structure to evaluate the presence and degree of differentiation of sub-populations of Atlantic Bluefin Tuna.
2012
VIII Incontro dei Dottorandi in Ecologia e Scienze dei Sistemi Acquatici Abstract book
74
74
Genetic structure, demographic trend and traceability of Atlantic and Mediterranean populations of Thunnus thynnus / Pintus E.; Cariani A.; Tinti F.; GBYP-PHASE 2 Consortium. - STAMPA. - (2012), pp. 74-74. (Intervento presentato al convegno VIII Incontro Dottorandi in Ecologia e Scienze dei Sistemi Acquatici tenutosi a Roma, Italia nel 27 February – 1 March 2012).
Pintus E.; Cariani A.; Tinti F.; GBYP-PHASE 2 Consortium
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/409772
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact