C.Baroni1, M.G. Bazzocchi1, S. Marchionni1,R.Bedini2, R.Pecci2, D.J.Manton3 1Dental School, Alma Mater Studiorum, University of Bologna, Bologna – Italy 2ISS, Rome Italy 3Dental School, University of Melbourne, Australia ID#: 177 Objectives To investigate, ex vivo, changes in morphology and mineral composition of mineral deficient hypomineralised molar enamel (MIH) after supplementation with a calcium-phosphate fluoride product (MI Paste Plus™, MIP, 10% CPP-ACP, 900ppm NaF,,GC Europe, Leuven, Belgium). In a previous study the product with no fluoride showed significant results only after a 2 years administration. Fluoride addiction should increase mineralization speed as tested here by SEM, EDX and micro-CT. Methods Enamel fragments (N=15) from newly erupted hypomineralised molar cusps after PEB (post-eruptive break-down) were taken from 6-7 year-old children (T0). Further samples (N=15) were taken after application of MI Paste five times daily for six months (T1). The fragments were analysed using μ-CT and ESEM-EDX (500X, 5000X); Anova Test was applied. Following the EDX analysis, two samples were goldpalladium sputtered: T0 before treatment, T1 after six months and were observed by SEM at 20000X magnifications. Conclusions Addition of 10% Sodium Fluoride to Calcium supplementation increses mineralization speed in MIH molars as shown by EDX peaks of calcium, and phosphate after six months. Micro-CT analysis visually but not statistically confirmed incresead mineralization. Baroni C., Marchionni S. MIH Supplementation Strategies : Prospective Clinical and Laboratory Trial. J.Dent.Res 2011 90: 371 Baroni C, Marchionni S, Bazzocchi MG, Cadenaro M, Nucci C, Manton DJ. A SEM and non-contact surface white light profilometry in vivo study of the effect of a crème containing CPP-ACP and fluoride on young etched enamel. Scanning. 2013 Jul 10. doi: 10.1002/sca.21102 Fig. 1: enamel fragments taken from the most damaged MIH cusps. Fig. 4 Six samples at T0 and six samples at T1 were used for micro-CT analysis, using a SkyScan 1072 Micro-CT (Bruker-microCT, Kontich, Belgium) NRecon V1.4.0 reconstruction software (Bruker, Kontich, Belgium). For every single microCT slice, a Region Of Interest (ROI) was chosen, each containing a whole single fragment. ROI allows for the calculation of the respective Volume Of Interest (VOI) for three different increasing acquisition ranges of Linear Coefficient of Attenuation (LAC) X-ray spectrum (proportional to X-ray permeability of the material), through enamel. Finally, a percentage alteration of the obtained volumes was calculated for the three different ranges. a), b) Untreated MIH enamel (T0) fragments: 2 layers of gray are visible due to different enamel linear coefficient of attenuation, LAC, (proportional to density). c), d) CPP-ACFP treated MIH enamel (T1) fragments, show a third lighter gray area (black circle) suggesting an increasing mineralisation. Fig. 2 ESEM-EDX results. a) T0: 500X, image and spectrum of the area shows a C peak higher than normal. b) T1: 500X, image and spectrum, after 6 months of CPP-ACFP treatment, show the Ca peak increased and C decreased. c) Mineral percentages concentration graph shows a significant (p<0.05) calcium increase and carbon decrease after the treatment, semiquantitative mineral percentages concentration are reported in the table below graph. Fig. 3 SEM analysis. c) T0 Protrusion of pointy prisms from a layer with no established framework. d) T1 Crystal elongation along the c-axis is initiated by deposition of small protrusions on the basal plane. Results T0 V1 V2 V3 V4 V1/V4 V2/V4 V3/V4 T0 0,89 ± 0,66 0,75 ± 0,77 0,00 ± 0,00 1,62 ± 1,26 56,20 43,70 0,10 T1 1,86 ± 1,94 2,31 ± 3,80 0,02 ± 0,03 4,15 ± 5,47 46,30 56,10 0,50 T1 T0 T0 T1 T0 T1
Fluoride added calcium supplementation to hypomineralized enamel
BARONI, CHIARA;MARCHIONNI, SILVIA;
2014
Abstract
C.Baroni1, M.G. Bazzocchi1, S. Marchionni1,R.Bedini2, R.Pecci2, D.J.Manton3 1Dental School, Alma Mater Studiorum, University of Bologna, Bologna – Italy 2ISS, Rome Italy 3Dental School, University of Melbourne, Australia ID#: 177 Objectives To investigate, ex vivo, changes in morphology and mineral composition of mineral deficient hypomineralised molar enamel (MIH) after supplementation with a calcium-phosphate fluoride product (MI Paste Plus™, MIP, 10% CPP-ACP, 900ppm NaF,,GC Europe, Leuven, Belgium). In a previous study the product with no fluoride showed significant results only after a 2 years administration. Fluoride addiction should increase mineralization speed as tested here by SEM, EDX and micro-CT. Methods Enamel fragments (N=15) from newly erupted hypomineralised molar cusps after PEB (post-eruptive break-down) were taken from 6-7 year-old children (T0). Further samples (N=15) were taken after application of MI Paste five times daily for six months (T1). The fragments were analysed using μ-CT and ESEM-EDX (500X, 5000X); Anova Test was applied. Following the EDX analysis, two samples were goldpalladium sputtered: T0 before treatment, T1 after six months and were observed by SEM at 20000X magnifications. Conclusions Addition of 10% Sodium Fluoride to Calcium supplementation increses mineralization speed in MIH molars as shown by EDX peaks of calcium, and phosphate after six months. Micro-CT analysis visually but not statistically confirmed incresead mineralization. Baroni C., Marchionni S. MIH Supplementation Strategies : Prospective Clinical and Laboratory Trial. J.Dent.Res 2011 90: 371 Baroni C, Marchionni S, Bazzocchi MG, Cadenaro M, Nucci C, Manton DJ. A SEM and non-contact surface white light profilometry in vivo study of the effect of a crème containing CPP-ACP and fluoride on young etched enamel. Scanning. 2013 Jul 10. doi: 10.1002/sca.21102 Fig. 1: enamel fragments taken from the most damaged MIH cusps. Fig. 4 Six samples at T0 and six samples at T1 were used for micro-CT analysis, using a SkyScan 1072 Micro-CT (Bruker-microCT, Kontich, Belgium) NRecon V1.4.0 reconstruction software (Bruker, Kontich, Belgium). For every single microCT slice, a Region Of Interest (ROI) was chosen, each containing a whole single fragment. ROI allows for the calculation of the respective Volume Of Interest (VOI) for three different increasing acquisition ranges of Linear Coefficient of Attenuation (LAC) X-ray spectrum (proportional to X-ray permeability of the material), through enamel. Finally, a percentage alteration of the obtained volumes was calculated for the three different ranges. a), b) Untreated MIH enamel (T0) fragments: 2 layers of gray are visible due to different enamel linear coefficient of attenuation, LAC, (proportional to density). c), d) CPP-ACFP treated MIH enamel (T1) fragments, show a third lighter gray area (black circle) suggesting an increasing mineralisation. Fig. 2 ESEM-EDX results. a) T0: 500X, image and spectrum of the area shows a C peak higher than normal. b) T1: 500X, image and spectrum, after 6 months of CPP-ACFP treatment, show the Ca peak increased and C decreased. c) Mineral percentages concentration graph shows a significant (p<0.05) calcium increase and carbon decrease after the treatment, semiquantitative mineral percentages concentration are reported in the table below graph. Fig. 3 SEM analysis. c) T0 Protrusion of pointy prisms from a layer with no established framework. d) T1 Crystal elongation along the c-axis is initiated by deposition of small protrusions on the basal plane. Results T0 V1 V2 V3 V4 V1/V4 V2/V4 V3/V4 T0 0,89 ± 0,66 0,75 ± 0,77 0,00 ± 0,00 1,62 ± 1,26 56,20 43,70 0,10 T1 1,86 ± 1,94 2,31 ± 3,80 0,02 ± 0,03 4,15 ± 5,47 46,30 56,10 0,50 T1 T0 T0 T1 T0 T1I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
