Olive tree leaves are a source of bioactive compounds, as oleuropein, flavonoids, and triterpenes. Hibiscus, used for centuries in the treatment of hypertension and hyperlipidemia, is known to be a rich source of anthocyanins. We evaluated the cardiovascular protective effects of Olea Europea leaves (OE) and Hibiscus flower (HE) extracts and a 26:4 w/w combination of the two (MIX). The extracts were characterized in their bioactive composition by HPLC-DAD-MS analysis. OE, HE and MIX reduced intracellular reactive oxygen species formation and improved cell viability following oxidative stress in dose dependent manner in HUVEC cell cultures. In isolated guinea pig left and right atria and aorta the effect on cardiovascular parameters was evaluated. OE, HE and MIX showed a similar negative inotropic effect. MIX, in contrast to OE and HE, showed a significant negative chronotropic activity. On vascular smooth muscle, OE and HE reduced the contraction induced by 80 mM K+ in a dose-dependent manner and MIX evidenced a miorelaxant activity higher than OE and HE. So, the combination of OE and HE could represent a nutraceutical approach in the protection of cardio and vascular health.
M. Malaguti, M. Micucci, T. Gallina Toschi, P. Comandini, R. Budriesi, S. Hrelia (2014). Cardio and vascular protective activities of Olea Europea and Hibiscus extratcs. Pisa : Pisa University Press.
Cardio and vascular protective activities of Olea Europea and Hibiscus extratcs
MALAGUTI, MARCO;MICUCCI, MATTEO;GALLINA TOSCHI, TULLIA;BUDRIESI, ROBERTA;HRELIA, SILVANA
2014
Abstract
Olive tree leaves are a source of bioactive compounds, as oleuropein, flavonoids, and triterpenes. Hibiscus, used for centuries in the treatment of hypertension and hyperlipidemia, is known to be a rich source of anthocyanins. We evaluated the cardiovascular protective effects of Olea Europea leaves (OE) and Hibiscus flower (HE) extracts and a 26:4 w/w combination of the two (MIX). The extracts were characterized in their bioactive composition by HPLC-DAD-MS analysis. OE, HE and MIX reduced intracellular reactive oxygen species formation and improved cell viability following oxidative stress in dose dependent manner in HUVEC cell cultures. In isolated guinea pig left and right atria and aorta the effect on cardiovascular parameters was evaluated. OE, HE and MIX showed a similar negative inotropic effect. MIX, in contrast to OE and HE, showed a significant negative chronotropic activity. On vascular smooth muscle, OE and HE reduced the contraction induced by 80 mM K+ in a dose-dependent manner and MIX evidenced a miorelaxant activity higher than OE and HE. So, the combination of OE and HE could represent a nutraceutical approach in the protection of cardio and vascular health.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
