Food bioactive peptides are currently investigated as they display functional activities for health promotion. Although high in carbohydrates, cereals contain substantial amount of proteins thus representing a possible dietary source of bioactive peptides. In the search of bioactive peptides in different wheat varieties a 7 kDa peptide was observed by means of HPLC-UV and nanoLC-nanoESI-QTof analyses in wheat flour extracts. Through data base searches the 7 kDa signal was ascribed to a Non-specific lipid-transfer protein (NsLTP) type 2 (sequence coverage of 92%). As no data regarding NsLTP type 2 biological activity in mammalian cells are present in the literature, experiments were aimed at the analysis of potential mechanisms by which this peptide can contribute to cardiovascular health using HUVEC cell cultures. In particular antioxidant activity and cytoprotective effects following oxidative or inflammatory stimulation were evaluated. Moreover, NsLTP type2 was also analysed for its ability to inhibit Angiotensin converting enzyme, a therapeutic target for the treatment of hypertension. This work was supported by BACCHUS project (FP7 European Commission Grant Agreement 312090).
E. Leoncini, G. Dinelli, C. Prata, L. Massaccesi, M. Malaguti, V. Bregola, et al. (2014). Bioactive peptides with cardiovascular protective activity from wheat grains. Pisa : Pisa University Press.
Bioactive peptides with cardiovascular protective activity from wheat grains
LEONCINI, EMANUELA;DINELLI, GIOVANNI;PRATA, CECILIA;MASSACCESI, LUCA;MALAGUTI, MARCO;BREGOLA, VALERIA;BOSI, SARA;FIORI, JESSICA;GOTTI, ROBERTO;HRELIA, SILVANA
2014
Abstract
Food bioactive peptides are currently investigated as they display functional activities for health promotion. Although high in carbohydrates, cereals contain substantial amount of proteins thus representing a possible dietary source of bioactive peptides. In the search of bioactive peptides in different wheat varieties a 7 kDa peptide was observed by means of HPLC-UV and nanoLC-nanoESI-QTof analyses in wheat flour extracts. Through data base searches the 7 kDa signal was ascribed to a Non-specific lipid-transfer protein (NsLTP) type 2 (sequence coverage of 92%). As no data regarding NsLTP type 2 biological activity in mammalian cells are present in the literature, experiments were aimed at the analysis of potential mechanisms by which this peptide can contribute to cardiovascular health using HUVEC cell cultures. In particular antioxidant activity and cytoprotective effects following oxidative or inflammatory stimulation were evaluated. Moreover, NsLTP type2 was also analysed for its ability to inhibit Angiotensin converting enzyme, a therapeutic target for the treatment of hypertension. This work was supported by BACCHUS project (FP7 European Commission Grant Agreement 312090).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
