The induction of apoptosis in the human enterocyte-like Caco-2 cell line mediated by three Bifidobacterium longum strains, which displays different adhesion properties, was investigated. Methods and Results: The three strains tested were defined for their adhesion properties using adhesion and autoaggregation assays. Strain B12 shows strong autoaggregation and strong adherence to Caco-2 cells; strain B18 shows strong autoaggregation and intermediate adherence; strain B2990 shows poor autoaggregation and poor adherence. Cocultures of Caco-2 cells with B. longum resulted in the induction of apoptosis, determined by DNA fragmentation test, only when autoaggregating or strongly adherent strains were used (B12 and B18). Conclusion: These results indicate that the interaction with aggregating/adherent probiotics can induce apoptosis of human colon epithelial cells, suggesting a role for these bacteria in the regulation of the cellular homeostasis of the gastrointestinal tract. Significance and Impact of the Study: B. longum as a probiotic is known for his beneficial effects to the human gut; the knowledge of its immunomodulatory and antitumor activities can be crucial for the healthcare and the prevention of cancer

Role of Bifidobacterium longum in the induction of apoptotic deletion in the human enterocyte-like Caco-2 cell line

NISSEN, LORENZO;PASINI, LUCA;BIAVATI, BRUNO;MALAGOLINI, NADIA;DALL'OLIO, FABIO;DELLA VALLE, GIULIANO;SGORBATI, BARBARA
2006

Abstract

The induction of apoptosis in the human enterocyte-like Caco-2 cell line mediated by three Bifidobacterium longum strains, which displays different adhesion properties, was investigated. Methods and Results: The three strains tested were defined for their adhesion properties using adhesion and autoaggregation assays. Strain B12 shows strong autoaggregation and strong adherence to Caco-2 cells; strain B18 shows strong autoaggregation and intermediate adherence; strain B2990 shows poor autoaggregation and poor adherence. Cocultures of Caco-2 cells with B. longum resulted in the induction of apoptosis, determined by DNA fragmentation test, only when autoaggregating or strongly adherent strains were used (B12 and B18). Conclusion: These results indicate that the interaction with aggregating/adherent probiotics can induce apoptosis of human colon epithelial cells, suggesting a role for these bacteria in the regulation of the cellular homeostasis of the gastrointestinal tract. Significance and Impact of the Study: B. longum as a probiotic is known for his beneficial effects to the human gut; the knowledge of its immunomodulatory and antitumor activities can be crucial for the healthcare and the prevention of cancer
2006
Nissen L.; Pasini L.; Biavati B.; Malagolini N.; Dall’Olio F.; Della Valle G.; Sgorbati B.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/32415
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