Gram-positive-staining, microaerophilic, non-spore-forming, fructose-6-phosphate phosphoketolase positive bacterial strains with a peculiar morphology were isolated from faecal samples of baby common marmoset (Callithrix jacchus). Cells of these strains showed a morphology never found in bifidobacterial species, which resembled a coiled snake, always coiled or ring shaped or forming a "Y" shape. MRM 3/1 and MRM 4/2 were chosen as representative strains and further characterized. The bacteria utilized a wide range of carbohydrates and produced urease. Glucose was fermented to acetate and lactate. The strain MRM 3/1 showed a peptidoglycan type unique among members of the genus Bifidobacterium . The DNA base composition was 64.7mol% G+C. Almost complete 16S rRNA, hsp60, clpC and rpoB gene sequences were obtained and phylogenetic relationships were determined. Comparative analysis of 16S rRNA gene sequences showed that strains MRM 3/1 and MRM 4/2 had the highest similarities to Bifidobacterium scardovii (DSM 13734T) (94.6%) and Bifidobacterium stellenboschense (DSMZ 23968T) (94.5%). Analysis of hsp60 showed that both strains were closely related to B. stellenboschense (97.5%), however, despite this high degree of similarity, our isolates could be distinguished from B. stellenboschense DSMZ 23968T also by low levels of DNA-DNA relatedness (30.4%). Therefore, strains MRM 3/1 and MRM 4/2 were located in an actinobacterial cluster and were more closely related to the genus Bifidobacterium than to other genera in Bifidobacteriaceae. On the basis of these results strains MRM 3/1T and MRM 4/2 represent a novel species within the genus Bifidobacterium, for which the name Bifidobacterium aesculapii sp. nov. is proposed; the type strain is MRM 3/1T (=DSM 26737T;= JCM 18761T).
M. Modesto, S. Michelini, I. Stefanini, A. Ferrara, S. Tacconi, B. Biavati, et al. (2014). Bifidobacterium aesculapii sp. nov., from the faeces of the baby common marmoset (Callithrix jacchus). INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64, 2819-2827 [10.1099/ijs.0.056937-0].
Bifidobacterium aesculapii sp. nov., from the faeces of the baby common marmoset (Callithrix jacchus)
MODESTO, MONICA MARIANNA;MICHELINI, SAMANTA;BIAVATI, BRUNO;MATTARELLI, PAOLA
2014
Abstract
Gram-positive-staining, microaerophilic, non-spore-forming, fructose-6-phosphate phosphoketolase positive bacterial strains with a peculiar morphology were isolated from faecal samples of baby common marmoset (Callithrix jacchus). Cells of these strains showed a morphology never found in bifidobacterial species, which resembled a coiled snake, always coiled or ring shaped or forming a "Y" shape. MRM 3/1 and MRM 4/2 were chosen as representative strains and further characterized. The bacteria utilized a wide range of carbohydrates and produced urease. Glucose was fermented to acetate and lactate. The strain MRM 3/1 showed a peptidoglycan type unique among members of the genus Bifidobacterium . The DNA base composition was 64.7mol% G+C. Almost complete 16S rRNA, hsp60, clpC and rpoB gene sequences were obtained and phylogenetic relationships were determined. Comparative analysis of 16S rRNA gene sequences showed that strains MRM 3/1 and MRM 4/2 had the highest similarities to Bifidobacterium scardovii (DSM 13734T) (94.6%) and Bifidobacterium stellenboschense (DSMZ 23968T) (94.5%). Analysis of hsp60 showed that both strains were closely related to B. stellenboschense (97.5%), however, despite this high degree of similarity, our isolates could be distinguished from B. stellenboschense DSMZ 23968T also by low levels of DNA-DNA relatedness (30.4%). Therefore, strains MRM 3/1 and MRM 4/2 were located in an actinobacterial cluster and were more closely related to the genus Bifidobacterium than to other genera in Bifidobacteriaceae. On the basis of these results strains MRM 3/1T and MRM 4/2 represent a novel species within the genus Bifidobacterium, for which the name Bifidobacterium aesculapii sp. nov. is proposed; the type strain is MRM 3/1T (=DSM 26737T;= JCM 18761T).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.