A PCR-RFLP technique has been applied on 13 species of Bifidobacterium in order to update a previous study carried out by Baffoni et al. [1]. This method is based on the restriction endonuclease activity of HaeIII on the PCR-amplified hsp60 partial gene sequence, and allows a rapid and efficient identification of Bifidobacterium spp. strains at species and subspecies level.
Identification of Bifidobacterium spp. using hsp60 PCR-RFLP analysis: An update / Verena Stenico;Samanta Michelini;Monica Modesto;Loredana Baffoni;Paola Mattarelli;Bruno Biavati. - In: ANAEROBE. - ISSN 1075-9964. - STAMPA. - 26:(2014), pp. 36-40. [10.1016/j.anaerobe.2013.12.004]
Identification of Bifidobacterium spp. using hsp60 PCR-RFLP analysis: An update
STENICO, VERENA;MICHELINI, SAMANTA;MODESTO, MONICA MARIANNA;BAFFONI, LOREDANA;MATTARELLI, PAOLA;BIAVATI, BRUNO
2014
Abstract
A PCR-RFLP technique has been applied on 13 species of Bifidobacterium in order to update a previous study carried out by Baffoni et al. [1]. This method is based on the restriction endonuclease activity of HaeIII on the PCR-amplified hsp60 partial gene sequence, and allows a rapid and efficient identification of Bifidobacterium spp. strains at species and subspecies level.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
