A PCR-RFLP technique has been applied on 13 species of Bifidobacterium in order to update a previous study carried out by Baffoni et al. [1]. This method is based on the restriction endonuclease activity of HaeIII on the PCR-amplified hsp60 partial gene sequence, and allows a rapid and efficient identification of Bifidobacterium spp. strains at species and subspecies level.
Verena Stenico, Samanta Michelini, Monica Modesto, Loredana Baffoni, Paola Mattarelli, Bruno Biavati (2014). Identification of Bifidobacterium spp. using hsp60 PCR-RFLP analysis: An update. ANAEROBE, 26, 36-40 [10.1016/j.anaerobe.2013.12.004].
Identification of Bifidobacterium spp. using hsp60 PCR-RFLP analysis: An update
STENICO, VERENA;MICHELINI, SAMANTA;MODESTO, MONICA MARIANNA;BAFFONI, LOREDANA;MATTARELLI, PAOLA;BIAVATI, BRUNO
2014
Abstract
A PCR-RFLP technique has been applied on 13 species of Bifidobacterium in order to update a previous study carried out by Baffoni et al. [1]. This method is based on the restriction endonuclease activity of HaeIII on the PCR-amplified hsp60 partial gene sequence, and allows a rapid and efficient identification of Bifidobacterium spp. strains at species and subspecies level.File in questo prodotto:
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