A PCR-RFLP technique has been applied on 13 species of Bifidobacterium in order to update a previous study carried out by Baffoni et al. [1]. This method is based on the restriction endonuclease activity of HaeIII on the PCR-amplified hsp60 partial gene sequence, and allows a rapid and efficient identification of Bifidobacterium spp. strains at species and subspecies level.
Identification of Bifidobacterium spp. using hsp60 PCR-RFLP analysis: An update
STENICO, VERENA;MICHELINI, SAMANTA;MODESTO, MONICA MARIANNA;BAFFONI, LOREDANA;MATTARELLI, PAOLA;BIAVATI, BRUNO
2014
Abstract
A PCR-RFLP technique has been applied on 13 species of Bifidobacterium in order to update a previous study carried out by Baffoni et al. [1]. This method is based on the restriction endonuclease activity of HaeIII on the PCR-amplified hsp60 partial gene sequence, and allows a rapid and efficient identification of Bifidobacterium spp. strains at species and subspecies level.File in questo prodotto:
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