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EnglishAn analytical method based on high-performance liquid chromatography (HPLC) combined with fluorescence detection (FL) has been developed for the simultaneous determination of fumonisin B1 (FB1) and its totally hydrolized metabolite aminopentol-1 (AP1) in pig liver. The sample preparation is based on a single solid phase extraction (SPE). o-Phthalaldehyde (OPA) was used for precolumn derivatization before the programmed reversed-phase analysis on phenylhexyl column. The developed method shows good repeatibility for inter- and intra-day precision as well as adequate linearity of calibration curves (r2 was 0.9855 for FB1 and 0.9831 for AP1). Average recoveries from the matrix were 93.6 % for FB1 and 95.3 % for AP1. The limit of quantification (LOQ) in swine liver was 75 µg/Kg for FB1 and 42 µg/Kg for AP1.
| Titolo: | Simple method for the simultaneous isolation and determination of fumonisin B1 and its metabolite aminopentol-1 in swine liver by liquid chromatography-fluorescence detection |
| Autore/i: | PAGLIUCA, GIAMPIERO; ZIRONI, ELISA; CECCOLINI A.; MATERA R.; SERRAZANETTI, GIAN PAOLO; PIVA, ANDREA |
| Autore/i Unibo: | |
| Anno: | 2005 |
| Rivista: | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.jchromb.2005.01.035 |
| Abstract: | An analytical method based on high-performance liquid chromatography (HPLC) combined with fluorescence detection (FL) has been developed for the simultaneous determination of fumonisin B1 (FB1) and its totally hydrolized metabolite aminopentol-1 (AP1) in pig liver. The sample preparation is based on a single solid phase extraction (SPE). o-Phthalaldehyde (OPA) was used for precolumn derivatization before the programmed reversed-phase analysis on phenylhexyl column. The developed method shows good repeatibility for inter- and intra-day precision as well as adequate linearity of calibration curves (r2 was 0.9855 for FB1 and 0.9831 for AP1). Average recoveries from the matrix were 93.6 % for FB1 and 95.3 % for AP1. The limit of quantification (LOQ) in swine liver was 75 µg/Kg for FB1 and 42 µg/Kg for AP1. |
| Data prodotto definitivo in UGOV: | 2005-10-10 13:08:05 |
| Appare nelle tipologie: | 1.01 Articolo in rivista |
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