An analytical method based on high-performance liquid chromatography (HPLC) combined with fluorescence detection (FL) has been developed for the simultaneous determination of fumonisin B1 (FB1) and its totally hydrolized metabolite aminopentol-1 (AP1) in pig liver. The sample preparation is based on a single solid phase extraction (SPE). o-Phthalaldehyde (OPA) was used for precolumn derivatization before the programmed reversed-phase analysis on phenylhexyl column. The developed method shows good repeatibility for inter- and intra-day precision as well as adequate linearity of calibration curves (r2 was 0.9855 for FB1 and 0.9831 for AP1). Average recoveries from the matrix were 93.6 % for FB1 and 95.3 % for AP1. The limit of quantification (LOQ) in swine liver was 75 µg/Kg for FB1 and 42 µg/Kg for AP1.

Simple method for the simultaneous isolation and determination of fumonisin B1 and its metabolite aminopentol-1 in swine liver by liquid chromatography-fluorescence detection

PAGLIUCA, GIAMPIERO;ZIRONI, ELISA;SERRAZANETTI, GIAN PAOLO;PIVA, ANDREA
2005

Abstract

An analytical method based on high-performance liquid chromatography (HPLC) combined with fluorescence detection (FL) has been developed for the simultaneous determination of fumonisin B1 (FB1) and its totally hydrolized metabolite aminopentol-1 (AP1) in pig liver. The sample preparation is based on a single solid phase extraction (SPE). o-Phthalaldehyde (OPA) was used for precolumn derivatization before the programmed reversed-phase analysis on phenylhexyl column. The developed method shows good repeatibility for inter- and intra-day precision as well as adequate linearity of calibration curves (r2 was 0.9855 for FB1 and 0.9831 for AP1). Average recoveries from the matrix were 93.6 % for FB1 and 95.3 % for AP1. The limit of quantification (LOQ) in swine liver was 75 µg/Kg for FB1 and 42 µg/Kg for AP1.
PAGLIUCA G.; ZIRONI E.; CECCOLINI A.; MATERA R.; SERRAZANETTI G.P.; PIVA A.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/2120
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