Most transcriptional regulators bind nucleotide motifs in the major groove, although some are able to recognize molecular determinants conferred by the minor groove of DNA. Here we report a transcrip- tional commutator switch that exploits the alternative readout of grooves to mediate opposite output regu- lation for the same input signal. This mechanism accounts for the ability of the Helicobacter pylori Fur regulator to repress the expression of both iron-inducible and iron-repressible genes. When iron is scarce, Fur binds to DNA as a dimer, through the readout of thymine pairs in the major groove, repressing iron-inducible transcription (FeON). Conversely, on iron-repressible elements the metal ion acts as corepressor, inducing Fur multimerization with consequent minor groove readout of AT-rich inverted repeats (FeOFF). Our results provide first evidence for a novel regulatory paradigm, in which the discriminative readout of DNA grooves enables to toggle between the repression of genes in a mutually exclusive manner.

FeON-FeOFF: the Helicobacter pylori Fur regulator commutates iron-responsive transcription by discriminative readout of opposed DNA grooves / F. Agriesti;D. Roncarati;F. Musiani;C. Del Campo;M. Iurlaro;F. Sparla;S. Ciurli;A. Danielli;V. Scarlato. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 42:5(2014), pp. 3138-3151. [10.1093/nar/gkt1258]

FeON-FeOFF: the Helicobacter pylori Fur regulator commutates iron-responsive transcription by discriminative readout of opposed DNA grooves

RONCARATI, DAVIDE;MUSIANI, FRANCESCO;SPARLA, FRANCESCA;CIURLI, STEFANO LUCIANO;DANIELLI, ALBERTO;SCARLATO, VINCENZO
2014

Abstract

Most transcriptional regulators bind nucleotide motifs in the major groove, although some are able to recognize molecular determinants conferred by the minor groove of DNA. Here we report a transcrip- tional commutator switch that exploits the alternative readout of grooves to mediate opposite output regu- lation for the same input signal. This mechanism accounts for the ability of the Helicobacter pylori Fur regulator to repress the expression of both iron-inducible and iron-repressible genes. When iron is scarce, Fur binds to DNA as a dimer, through the readout of thymine pairs in the major groove, repressing iron-inducible transcription (FeON). Conversely, on iron-repressible elements the metal ion acts as corepressor, inducing Fur multimerization with consequent minor groove readout of AT-rich inverted repeats (FeOFF). Our results provide first evidence for a novel regulatory paradigm, in which the discriminative readout of DNA grooves enables to toggle between the repression of genes in a mutually exclusive manner.
2014
FeON-FeOFF: the Helicobacter pylori Fur regulator commutates iron-responsive transcription by discriminative readout of opposed DNA grooves / F. Agriesti;D. Roncarati;F. Musiani;C. Del Campo;M. Iurlaro;F. Sparla;S. Ciurli;A. Danielli;V. Scarlato. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - STAMPA. - 42:5(2014), pp. 3138-3151. [10.1093/nar/gkt1258]
F. Agriesti;D. Roncarati;F. Musiani;C. Del Campo;M. Iurlaro;F. Sparla;S. Ciurli;A. Danielli;V. Scarlato
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/207626
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