Growing evidence supports the critical role of lipid peroxidation products in the control of cell proliferation.In previous studies we demonstrated the e fficient restriction of the proliferation rate in several cell lines resulting from the in vitro treatment with endogenous lipid polar components of cell membranes.Among these,9-hydroxystearic acid (9-HSA),a primary intermediate of lipid peroxidation,induced a signi ficant arrest in G0/G1 in HT29 colon cancer cells.In response to 9-HSA treatment of HT29 we observed cell growth arrest and increase in p21 WAF1 expression both at the transcriptional and the translational levels.Growth of p21 WAF1 -deleted HCT116 human colon carcinoma cells was not inhibited by 9-HSA.We present evidence that p21 WAF1 is required for 9-HSA mediated growth arrest in human colon carcinoma cells. 2003 Elsevier Inc.All rights reserved.

9-Hydroxystearic acid upregulates p21WAF1 in HT29 cancer cells.

CALONGHI, NATALIA;CAPPADONE, CONCETTINA;PAGNOTTA, ELEONORA;FARRUGGIA, GIOVANNA;BUONTEMPO, FRANCESCA;BOGA, CARLA;SANTUCCI, MARIA ALESSANDRA;MASOTTI, LANFRANCO
2004

Abstract

Growing evidence supports the critical role of lipid peroxidation products in the control of cell proliferation.In previous studies we demonstrated the e fficient restriction of the proliferation rate in several cell lines resulting from the in vitro treatment with endogenous lipid polar components of cell membranes.Among these,9-hydroxystearic acid (9-HSA),a primary intermediate of lipid peroxidation,induced a signi ficant arrest in G0/G1 in HT29 colon cancer cells.In response to 9-HSA treatment of HT29 we observed cell growth arrest and increase in p21 WAF1 expression both at the transcriptional and the translational levels.Growth of p21 WAF1 -deleted HCT116 human colon carcinoma cells was not inhibited by 9-HSA.We present evidence that p21 WAF1 is required for 9-HSA mediated growth arrest in human colon carcinoma cells. 2003 Elsevier Inc.All rights reserved.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
N. CALONGHI; C. CAPPADONE; E. PAGNOTTA; G. FARRUGGIA; F. BUONTEMPO; C. BOGA; G.L. BRUSA; M.A. SANTUCCI; MASOTTI L.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/2022
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