Growing evidence supports the critical role of lipid peroxidation products in the control of cell proliferation.In previous studies we demonstrated the e fficient restriction of the proliferation rate in several cell lines resulting from the in vitro treatment with endogenous lipid polar components of cell membranes.Among these,9-hydroxystearic acid (9-HSA),a primary intermediate of lipid peroxidation,induced a signi ficant arrest in G0/G1 in HT29 colon cancer cells.In response to 9-HSA treatment of HT29 we observed cell growth arrest and increase in p21 WAF1 expression both at the transcriptional and the translational levels.Growth of p21 WAF1 -deleted HCT116 human colon carcinoma cells was not inhibited by 9-HSA.We present evidence that p21 WAF1 is required for 9-HSA mediated growth arrest in human colon carcinoma cells. 2003 Elsevier Inc.All rights reserved.
Calonghi, N., Cappadone, C., Pagnotta, E., Farruggia, G., Buontempo, F., Boga, C., et al. (2004). 9-Hydroxystearic acid upregulates p21WAF1 in HT29 cancer cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 314, 138-142 [10.1016/j.bbrc.2003.12.066].
9-Hydroxystearic acid upregulates p21WAF1 in HT29 cancer cells.
CALONGHI, NATALIA;CAPPADONE, CONCETTINA;PAGNOTTA, ELEONORA;FARRUGGIA, GIOVANNA;BUONTEMPO, FRANCESCA;BOGA, CARLA;SANTUCCI, MARIA ALESSANDRA;MASOTTI, LANFRANCO
2004
Abstract
Growing evidence supports the critical role of lipid peroxidation products in the control of cell proliferation.In previous studies we demonstrated the e fficient restriction of the proliferation rate in several cell lines resulting from the in vitro treatment with endogenous lipid polar components of cell membranes.Among these,9-hydroxystearic acid (9-HSA),a primary intermediate of lipid peroxidation,induced a signi ficant arrest in G0/G1 in HT29 colon cancer cells.In response to 9-HSA treatment of HT29 we observed cell growth arrest and increase in p21 WAF1 expression both at the transcriptional and the translational levels.Growth of p21 WAF1 -deleted HCT116 human colon carcinoma cells was not inhibited by 9-HSA.We present evidence that p21 WAF1 is required for 9-HSA mediated growth arrest in human colon carcinoma cells. 2003 Elsevier Inc.All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
