Folates – the natural chemically reduced forms of folic acid (vitamin B9) - are cofactors in essential metabolic pathways such as DNA synthesis and methylation pathways. Humans cannot synthesize folate and depend on intake both from the diet (green vegetables, cereals, rice, milk, fermented milk products, etc.) and from indigenous folate synthesizing bacteria of the intestinal microbiota. Low folate levels increase the risk for neural tube defects and may increase the risk for e.g. certain cancer forms, cardiovascular disease and Alzheimer’s. Screening for folate production of the bifidobacteria isolates from human adult and infant (1-6 month old) was performed. Strains typical of infants, such as Bifidobacterium longum subsp. infantis and B. breve, and of adults ( B. adolescentis) were selected for characterization. The aim of the present work was to investigate bifidobacteria from human host of different age with different feeding habits in order to establish a possible correlation between diet and the folate production. Folate is present in many different forms in humans. The detectable forms studied in the present work are 5-CH3-H4, H4 and total folate content. Bifidobacteria strains were cultivated in folate free synthetic media. Validated HPLC method was used to analyze deconjugated folates extracted from bacterial biomass.
D'Aimmo M.R., Modesto M., Mattarelli P., Sgorbati B., Biavati B., Andlid T. (2013). FOLATE PRODUCTION IN BIFIDOBACTERIA FROM INFANT AND ADULT HUMANS. UNITED EUROPEAN GASTROENTEROLOGY JOURNAL, 1(Supp. 1), A75-A75.
FOLATE PRODUCTION IN BIFIDOBACTERIA FROM INFANT AND ADULT HUMANS
MODESTO, MONICA MARIANNA;MATTARELLI, PAOLA;SGORBATI, BARBARA;BIAVATI, BRUNO;
2013
Abstract
Folates – the natural chemically reduced forms of folic acid (vitamin B9) - are cofactors in essential metabolic pathways such as DNA synthesis and methylation pathways. Humans cannot synthesize folate and depend on intake both from the diet (green vegetables, cereals, rice, milk, fermented milk products, etc.) and from indigenous folate synthesizing bacteria of the intestinal microbiota. Low folate levels increase the risk for neural tube defects and may increase the risk for e.g. certain cancer forms, cardiovascular disease and Alzheimer’s. Screening for folate production of the bifidobacteria isolates from human adult and infant (1-6 month old) was performed. Strains typical of infants, such as Bifidobacterium longum subsp. infantis and B. breve, and of adults ( B. adolescentis) were selected for characterization. The aim of the present work was to investigate bifidobacteria from human host of different age with different feeding habits in order to establish a possible correlation between diet and the folate production. Folate is present in many different forms in humans. The detectable forms studied in the present work are 5-CH3-H4, H4 and total folate content. Bifidobacteria strains were cultivated in folate free synthetic media. Validated HPLC method was used to analyze deconjugated folates extracted from bacterial biomass.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
