Plant regeneration is a critical step in most in vitro breeding techniques. The present paper studies the effects of a low-molecular-weight humic acid (HA) on morphogenesis from pear and quince leaf explants. Variable HA amounts [0 (control), 1, 5, 10 and 20 mg C L-1] were added to the regeneration media. A dose-response effect was observed in pear for root and shoot production; it was improved at HA 1 mg C L-1 and considerably reduced at the highest amounts. HA was, instead, ineffective in quince. The 1H HR-MAS NMR analyses of calli in the induction phase showed more evident metabolite (asparagine, alanine and γ-amino butyric acid) signals in quince than pear. The assignment of overlapped signals in both genotypes were supported by the 2D NMR analyses. Spectroscopic characterization suggested also an enhancement of asparagine contents in morphogenic calli of pear with respect to the control and higher HA amount treatments.
Grazia Marino, Valeria Righi, Andrea Simoni, Luisa Schenetti, Adele Mucci, Vitaliano Tugnoli, et al. (2013). Effect of a peat humic acid on morphogenesis in leaf explants of Pyrus communis and Cydonia oblonga. Metabolomic analysis at an early stage of regeneration. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 61(21), 4979-4987 [10.1021/jf4004785].
Effect of a peat humic acid on morphogenesis in leaf explants of Pyrus communis and Cydonia oblonga. Metabolomic analysis at an early stage of regeneration
MARINO, GRAZIA;RIGHI, VALERIA;SIMONI, ANDREA;TUGNOLI, VITALIANO;MUZZI, ENRICO;FRANCIOSO, ORNELLA
2013
Abstract
Plant regeneration is a critical step in most in vitro breeding techniques. The present paper studies the effects of a low-molecular-weight humic acid (HA) on morphogenesis from pear and quince leaf explants. Variable HA amounts [0 (control), 1, 5, 10 and 20 mg C L-1] were added to the regeneration media. A dose-response effect was observed in pear for root and shoot production; it was improved at HA 1 mg C L-1 and considerably reduced at the highest amounts. HA was, instead, ineffective in quince. The 1H HR-MAS NMR analyses of calli in the induction phase showed more evident metabolite (asparagine, alanine and γ-amino butyric acid) signals in quince than pear. The assignment of overlapped signals in both genotypes were supported by the 2D NMR analyses. Spectroscopic characterization suggested also an enhancement of asparagine contents in morphogenic calli of pear with respect to the control and higher HA amount treatments.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
