Disinfection of electrospun materials is commonly carried out by ethanol (EtOH) soaking. However, in the literature no systematic investigation on the effect of EtOH disinfection on scaffold properties is available. In this study electrospun poly(L-lactic acid) (PLLA) scaffolds were differently treated in EtOH and changes of fiber morphology, fiber surface topography and scaffold thermo-mechanical properties were evaluated. It was found that EtOH induced the formation of an amount of crystal phase, in the initially amorphous fibers, which depends on EtOH grade, soaking time and temperature. Completely amorphous PLLA (a-PLLA) and semicrystalline PLIA (sc-PLLA) scaffolds were produced by applying different EtOH treatments to as-spun PLLA scaffold. Compared to a-PLLA, sc-PLLA was stiffer and composed of fibers with rougher surface. Cell culture performed by using permanent cell line H9c2 demonstrated that changes of scaffold properties determined a different cell response, in particular in the expression of proteins correlated to cell motility and cell adhesion.

C. Gualandi, M. Govoni, L. Foroni, S. Valente, M. Bianchi, E. Giordano, et al. (2012). Ethanol disinfection affects physical properties and cell response of electrospun poly(L-lactic acid) scaffolds. EUROPEAN POLYMER JOURNAL, 48, 2008-2018 [10.1016/j.eurpolymj.2012.09.016].

Ethanol disinfection affects physical properties and cell response of electrospun poly(L-lactic acid) scaffolds

GUALANDI, CHIARA;GOVONI, MARCO;VALENTE, SABRINA;GIORDANO, EMANUELE DOMENICO;PASQUINELLI, GIANANDREA;FOCARETE, MARIA LETIZIA
2012

Abstract

Disinfection of electrospun materials is commonly carried out by ethanol (EtOH) soaking. However, in the literature no systematic investigation on the effect of EtOH disinfection on scaffold properties is available. In this study electrospun poly(L-lactic acid) (PLLA) scaffolds were differently treated in EtOH and changes of fiber morphology, fiber surface topography and scaffold thermo-mechanical properties were evaluated. It was found that EtOH induced the formation of an amount of crystal phase, in the initially amorphous fibers, which depends on EtOH grade, soaking time and temperature. Completely amorphous PLLA (a-PLLA) and semicrystalline PLIA (sc-PLLA) scaffolds were produced by applying different EtOH treatments to as-spun PLLA scaffold. Compared to a-PLLA, sc-PLLA was stiffer and composed of fibers with rougher surface. Cell culture performed by using permanent cell line H9c2 demonstrated that changes of scaffold properties determined a different cell response, in particular in the expression of proteins correlated to cell motility and cell adhesion.
2012
C. Gualandi, M. Govoni, L. Foroni, S. Valente, M. Bianchi, E. Giordano, et al. (2012). Ethanol disinfection affects physical properties and cell response of electrospun poly(L-lactic acid) scaffolds. EUROPEAN POLYMER JOURNAL, 48, 2008-2018 [10.1016/j.eurpolymj.2012.09.016].
C. Gualandi; M. Govoni; L. Foroni; S. Valente; M. Bianchi; E. Giordano; G. Pasquinelli; F. Biscarini; M.L. Focarete
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/130536
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 45
  • ???jsp.display-item.citation.isi??? 44
social impact