Three different olfactometers were evaluated in order to develop a bioassay procedure testing for the olfactory responses of Cydia molesta. Females were tested individually using a linear and a Y-tube olfactometer, and in groups using a dual-choice arena. Room temperature, relative humidity, light intensity, airflow, age of the moths and odour source were kept constant during experiments. The odour source tested was a green shoot of peach excised 10 min before experiments started. Cydia molesta females showed a significant response to this plant odour in all olfactometers. A number of qualitative aspects were found to be in favour of the dual choice arena as a tool for screening potentially attractive odour sources. It allowed for a differentiation of the response of mated and virgin females. Experimental conditions allowed the circadian rhythm of insects to be mimicked. Manipulation of individuals is reduced and flight is not precluded.
Bioassay approaches to observing behavioural responses of adult females Cydia molesta to host plant odour
PASQUALINI, EDISON;
2004
Abstract
Three different olfactometers were evaluated in order to develop a bioassay procedure testing for the olfactory responses of Cydia molesta. Females were tested individually using a linear and a Y-tube olfactometer, and in groups using a dual-choice arena. Room temperature, relative humidity, light intensity, airflow, age of the moths and odour source were kept constant during experiments. The odour source tested was a green shoot of peach excised 10 min before experiments started. Cydia molesta females showed a significant response to this plant odour in all olfactometers. A number of qualitative aspects were found to be in favour of the dual choice arena as a tool for screening potentially attractive odour sources. It allowed for a differentiation of the response of mated and virgin females. Experimental conditions allowed the circadian rhythm of insects to be mimicked. Manipulation of individuals is reduced and flight is not precluded.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
