Oat (Avena sativa L.) is an important crop produced in various regions of Europe and North America. Oat lipids are a heterogeneous mixture of acyl lipids and unsaponifiable components. The neutral lipids are mainly triacylglycerols (TAGs) and account for 50–60% of total oat lipids. Oat oil is also rich in polar lipids; that is, phospholipids (6–26%) and glycolipids (6–17%). Characterization of oat polar lipids has largely been performed by thin-layer chromatography, but the characterization of phospholipid classes has been poorly studied. The aim of our work was the determination of different phospholipids in Romanian oat samples. To this end, one commercial sample (Comun) and four pure varieties (Jeremy, Lovrin 1, Lovrin 27-T and Mures) were used. HPLC-ELSD results allowed to establish that phosphatidylglycerol (PG), phosphatidylcholine (PC), and lyso-PC were the most representative phospholipids in all the oat samples, except for Mures variety where phosphatidylethanolamine (PE), PC, and lyso-PC were the principal phospholipids. Additionally, HPLC-ESI MS analysis showed that C16:0, C16:1, C18:0, C18:1, C18:2, C18:3, C20:0 and C20:1 were the fatty acids bounded to the phospholipid glycerol chain.
Determination of phospholipids in oat samples by HPLC-ELSD and HPLC-ESI-MS / C. Montealegre; V. Verardo; A.M. Gomez-Caravaca; C. García-Ruiz; M.L. Marina; M.F. Caboni. - STAMPA. - (2012), pp. 503-503. (Intervento presentato al convegno 36th ISCC and 9th GC×GC Symposium tenutosi a Riva del Garda nel May 27 – June 1, 2012).
Determination of phospholipids in oat samples by HPLC-ELSD and HPLC-ESI-MS
VERARDO, VITO;CABONI, MARIA
2012
Abstract
Oat (Avena sativa L.) is an important crop produced in various regions of Europe and North America. Oat lipids are a heterogeneous mixture of acyl lipids and unsaponifiable components. The neutral lipids are mainly triacylglycerols (TAGs) and account for 50–60% of total oat lipids. Oat oil is also rich in polar lipids; that is, phospholipids (6–26%) and glycolipids (6–17%). Characterization of oat polar lipids has largely been performed by thin-layer chromatography, but the characterization of phospholipid classes has been poorly studied. The aim of our work was the determination of different phospholipids in Romanian oat samples. To this end, one commercial sample (Comun) and four pure varieties (Jeremy, Lovrin 1, Lovrin 27-T and Mures) were used. HPLC-ELSD results allowed to establish that phosphatidylglycerol (PG), phosphatidylcholine (PC), and lyso-PC were the most representative phospholipids in all the oat samples, except for Mures variety where phosphatidylethanolamine (PE), PC, and lyso-PC were the principal phospholipids. Additionally, HPLC-ESI MS analysis showed that C16:0, C16:1, C18:0, C18:1, C18:2, C18:3, C20:0 and C20:1 were the fatty acids bounded to the phospholipid glycerol chain.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
