Pelargonium zonate spot virus (PZSV) is the single member of the Anulavirus genus (Fam. Bromoviridae) first isolated from tomato in Italy and later reported also from Spain, France, USA and Israel. Up to now PZSV has known to naturally infect only herbaceous hosts as tomato, pepper, artichoke and common weeds often symptomless. Symptoms on leaves and fruits of infected tomato plants are characterized by line patterns, chlorotic and necrotic rings, together with plant stunting, leaf malformation and poor fruit set, which often result in plant death as infected cells show severe cytopathological alterations. The virus is transmitted by mechanical inoculation, grafting, and through seed by means of infected pollen carried on the bodies of thrips. In May 2011 plants of kiwifruit (Actinidia chinensis) cv. Hort16A, exhibiting viral symptoms, were observed in two orchards in Faenza province, Emilia-Romagna region. Symptoms include chlorotic and necrotic rings on leaves and depressed areas on the fruits with consequently deformation of the berries. The causal agent has been successfully transmitted to indicator plants, as Chenopodium quinoa, Nicotiana benthamiana, N. glutinosa and N. tabacum, by mechanical inoculation. Viral particles with diameter of about 30 nm were observed on viral purification obtained from C. quinoa infected leaves. Random amplification and sequencing of nucleic acids isolated from purified virus, allowed identification of a short segment showing high nucleotide identity with 5’ end of PZSV RNA2. RT-PCR analyses, performed using PZSV primer pair, specifically identified the virus in all indicator hosts and in leaves and fruits collected from all symptomatic kiwifruit plants. The new PZSV isolate has been characterized by sequencing and by ultrastructural and immuno-transmission electron microscopy investigations. Moreover, in order to determine the effect of the virus on cellular water compartmentation, tissue metabolic activity and overall quality, fruits and leaves from infected and healthy plants have been compared by NMR measurements (Proton transverse relaxation time), isothermal calo-respirometry (metabolic heat production) and visual appearance by computer vision system.
PELARGONIUM ZONATE SPOT VIRUS infecting kiwifruit plants in Italy. Book Abs ‘22 nd International Conf Virus Graft Transmissible Diseases Fruit Crops’, Roma, 3 -8 June 2012, p.27 / R Biccheri; AR Babini; A Blouin; C Lanzoni; A Pisi; C Poggi Pollini; R Credi; L Laghi; P Rocculi; C Rubies Autonell; MN Pearson; C Ratti. - STAMPA. - (2012), pp. 27-27. (Intervento presentato al convegno Book of abs of 22 nd International Conf Virus Graft Transmissible Diseases Fruit Crops tenutosi a ROMA nel 3-8 giugno 2012).
PELARGONIUM ZONATE SPOT VIRUS infecting kiwifruit plants in Italy. Book Abs ‘22 nd International Conf Virus Graft Transmissible Diseases Fruit Crops’, Roma, 3 -8 June 2012, p.27.
BICCHERI, ROBERTA;PISI, ANNAMARIA;POGGI POLLINI, CARLO;LAGHI, LUCA;ROCCULI, PIETRO;RUBIES AUTONELL, CONCEPCION;RATTI, CLAUDIO
2012
Abstract
Pelargonium zonate spot virus (PZSV) is the single member of the Anulavirus genus (Fam. Bromoviridae) first isolated from tomato in Italy and later reported also from Spain, France, USA and Israel. Up to now PZSV has known to naturally infect only herbaceous hosts as tomato, pepper, artichoke and common weeds often symptomless. Symptoms on leaves and fruits of infected tomato plants are characterized by line patterns, chlorotic and necrotic rings, together with plant stunting, leaf malformation and poor fruit set, which often result in plant death as infected cells show severe cytopathological alterations. The virus is transmitted by mechanical inoculation, grafting, and through seed by means of infected pollen carried on the bodies of thrips. In May 2011 plants of kiwifruit (Actinidia chinensis) cv. Hort16A, exhibiting viral symptoms, were observed in two orchards in Faenza province, Emilia-Romagna region. Symptoms include chlorotic and necrotic rings on leaves and depressed areas on the fruits with consequently deformation of the berries. The causal agent has been successfully transmitted to indicator plants, as Chenopodium quinoa, Nicotiana benthamiana, N. glutinosa and N. tabacum, by mechanical inoculation. Viral particles with diameter of about 30 nm were observed on viral purification obtained from C. quinoa infected leaves. Random amplification and sequencing of nucleic acids isolated from purified virus, allowed identification of a short segment showing high nucleotide identity with 5’ end of PZSV RNA2. RT-PCR analyses, performed using PZSV primer pair, specifically identified the virus in all indicator hosts and in leaves and fruits collected from all symptomatic kiwifruit plants. The new PZSV isolate has been characterized by sequencing and by ultrastructural and immuno-transmission electron microscopy investigations. Moreover, in order to determine the effect of the virus on cellular water compartmentation, tissue metabolic activity and overall quality, fruits and leaves from infected and healthy plants have been compared by NMR measurements (Proton transverse relaxation time), isothermal calo-respirometry (metabolic heat production) and visual appearance by computer vision system.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
