Human femoral arteries were cultured up to 56 days. Samples were processed for light, immunohistochemical, and transmission electron microscopy. Arteries became rapidly depopulated; at day 42, an endothelial lining (CD31(+), Weibel-Palade bodies) developed on the intima; endothelium was in continuity with mesenchymal stromal cells (CD44(+), CD90(low), CD105(low)) placed on adventitia. The media-adventitia area showed heterogeneous cell populations. In long-term organ culture, femoral artery develops a continuous cell coverage that differentiates to endothelium on the intima exclusively. This suggests that distinct topographical factors, such as resident progenitors and/or matrix signals, are able to regulate vascular homeostasis in adult life.
Valente S, Panarese N, Buzzi M, Alberghini M, Stella A, Pasquinelli G. (2012). The emerging issue of human resident arterial progenitors: the contribution of organ culture. ULTRASTRUCTURAL PATHOLOGY, 36 (2), 117-123 [10.3109/01913123.2011.631726].
The emerging issue of human resident arterial progenitors: the contribution of organ culture.
VALENTE, SABRINA;STELLA, ANDREA;PASQUINELLI, GIANANDREA
2012
Abstract
Human femoral arteries were cultured up to 56 days. Samples were processed for light, immunohistochemical, and transmission electron microscopy. Arteries became rapidly depopulated; at day 42, an endothelial lining (CD31(+), Weibel-Palade bodies) developed on the intima; endothelium was in continuity with mesenchymal stromal cells (CD44(+), CD90(low), CD105(low)) placed on adventitia. The media-adventitia area showed heterogeneous cell populations. In long-term organ culture, femoral artery develops a continuous cell coverage that differentiates to endothelium on the intima exclusively. This suggests that distinct topographical factors, such as resident progenitors and/or matrix signals, are able to regulate vascular homeostasis in adult life.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
