The inhibitory power of three different active Nylon membranes, separately loaded with three different protease inhibitors, was studied with the aim of reducing the increased elastase concentration occurring during hemodialysis or extracorporeal blood circulation in patients undergoing cardiopulmonary bypass. Chemical grafting was carried out to make the inert Nylon membrane suitable for the immobilization of the inhibitors. The behavior of immobilized R1-antitrypsin, bovine pancreatic trypsin inhibitor (BPTI), or elastatinal was separately studied. R1-Antitrypsin and BPTI were covalently immobilized by means of a diazotization process, whereas elastatinal was covalently attached via a condensation process mediated by glutaraldehyde. The inhibitory power of each membrane type was studied as a function of the amount of immobilized inhibitor and temperature. All active membranes have shown good inhibitory power. The most efficient membrane was that loaded with R1-antitrypsin, the less efficient that with BPTI.

Reduction of active elastase concentration by means of immobilized inhibitors: a novel therapeutic approach / GRANO V.; TASCO G.; CASADIO R.; DIANO N.; PORTACCIO M.; ROSSI S.; BENCIVENGA U.; COMPIANI M.; DE MAIO A.; MITA D.G.. - In: BIOTECHNOLOGY PROGRESS. - ISSN 8756-7938. - STAMPA. - 20:(2004), pp. 968-974. [10.1021/bp034304b]

Reduction of active elastase concentration by means of immobilized inhibitors: a novel therapeutic approach

TASCO, GIANLUCA;CASADIO, RITA;
2004

Abstract

The inhibitory power of three different active Nylon membranes, separately loaded with three different protease inhibitors, was studied with the aim of reducing the increased elastase concentration occurring during hemodialysis or extracorporeal blood circulation in patients undergoing cardiopulmonary bypass. Chemical grafting was carried out to make the inert Nylon membrane suitable for the immobilization of the inhibitors. The behavior of immobilized R1-antitrypsin, bovine pancreatic trypsin inhibitor (BPTI), or elastatinal was separately studied. R1-Antitrypsin and BPTI were covalently immobilized by means of a diazotization process, whereas elastatinal was covalently attached via a condensation process mediated by glutaraldehyde. The inhibitory power of each membrane type was studied as a function of the amount of immobilized inhibitor and temperature. All active membranes have shown good inhibitory power. The most efficient membrane was that loaded with R1-antitrypsin, the less efficient that with BPTI.
2004
Reduction of active elastase concentration by means of immobilized inhibitors: a novel therapeutic approach / GRANO V.; TASCO G.; CASADIO R.; DIANO N.; PORTACCIO M.; ROSSI S.; BENCIVENGA U.; COMPIANI M.; DE MAIO A.; MITA D.G.. - In: BIOTECHNOLOGY PROGRESS. - ISSN 8756-7938. - STAMPA. - 20:(2004), pp. 968-974. [10.1021/bp034304b]
GRANO V.; TASCO G.; CASADIO R.; DIANO N.; PORTACCIO M.; ROSSI S.; BENCIVENGA U.; COMPIANI M.; DE MAIO A.; MITA D.G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/1189
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