Fingerprinting in Raspberry by SSRs Analysis and Cultivar Characterisation

Fruit quality, sustainable production and content of healthy compounds are the main objectives of many berry breeding programs. The conformity of nursery materials and correct correspondence of the cultivars is a key factor for growers and consumers. In commercial orchards, mislabelled soft fruit accessions are very common, and accurate DNA fingerprinting is useful to verify the trueness to type of the cultivars. A number of marker-based technologies have been set up to fingerprint each genotype at any stage of development and independently from environmental factors that may influence the phenotype. An applicative scheme for raspberry fingerprinting has been developed in order to verify the putative pedigree of several accessions derived from a breeding programme in which the cultivars ‘Tulameen’, ‘Tulameen Giallo’, ‘Polka’, ‘Autumn Bliss’, ‘Autumn Treasure’, ‘Sugana’ and ‘Ruby’ were used and derived genotypes characterised. In the present work some genotype showed the same allelic pattern (‘Tulameen’ and ‘Tulameen Giallo’; ‘Sugana’ and ‘Sugana Gialla’) and more advanced techniques as S-SAP could be used in the future.