Several analytical techniques, namely gas chromatography-mass spectrometry (GC-MS), proton transfer reaction-mass spectrometry (PTR-MS) and laser photoacoustic detection (LPD), were used to characterize the volatile emission by Erwinia amylovora and other plant-pathogenic bacteria. These techniques demonstrated that diverse volatiles are emitted by the different bacteria. The distinct blend of volatiles produced by bacteria allowed their identification via electronic nose. The present study report the results obtained using an electronic nose based on metal oxide semiconductor sensors for discriminating Erwinia amylovora from other plant associate bacteria. Two different approaches were used for identification. The first one was the direct comparison of the odorous profiles of unknown bacterial isolates with four selected reference species. The second approach was the use of previously developed data-bases representing the odorous variability among several bacterial species. Using these two strategies, the electronic-nose successfully identified the isolates in the 87.5% and 62.5% of the cases, respectively. Finally, the profiling of the volatiles emitted by Erwinia amylovora allowed to identify some metabolic markers with a biological activity in vitro.

Emission of volatile compounds by Erwinia amylovora: biological activity in vitro and possible exploitation for bacterial identification

SPINELLI, FRANCESCO;CELLINI, ANTONIO;RODRIGUEZ ESTRADA, MARIA TERESA;COSTA, GUGLIELMO;SAVIOLI, STEFANO;
2012

Abstract

Several analytical techniques, namely gas chromatography-mass spectrometry (GC-MS), proton transfer reaction-mass spectrometry (PTR-MS) and laser photoacoustic detection (LPD), were used to characterize the volatile emission by Erwinia amylovora and other plant-pathogenic bacteria. These techniques demonstrated that diverse volatiles are emitted by the different bacteria. The distinct blend of volatiles produced by bacteria allowed their identification via electronic nose. The present study report the results obtained using an electronic nose based on metal oxide semiconductor sensors for discriminating Erwinia amylovora from other plant associate bacteria. Two different approaches were used for identification. The first one was the direct comparison of the odorous profiles of unknown bacterial isolates with four selected reference species. The second approach was the use of previously developed data-bases representing the odorous variability among several bacterial species. Using these two strategies, the electronic-nose successfully identified the isolates in the 87.5% and 62.5% of the cases, respectively. Finally, the profiling of the volatiles emitted by Erwinia amylovora allowed to identify some metabolic markers with a biological activity in vitro.
F. Spinelli; A. Cellini; J. L. Vanneste; M. T. Rodriguez-Estrada; G. Costa; S. Savioli; F. J.M. Harren; S. M. Cristescu
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/108483
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