The analysis of the sequence of Helicobacter pylori UreD(H), an accessory protein involved in the activation of urease through the assembly of the Ni2+ - containing active site, revealed the presence of two domains. The structure of these domains was calculated using threading and modeling algorithms. A search for putative binding sites on the protein surface was carried out using dedicated algorithms sensitive to either sequence conservation or structural similarity based on geometry and physicochemical properties. The results suggest that UreD(H) acts as a multifunctional molecular recognition platform facilitating the interaction between apo-urease and the ancillary pro- teins UreG, UreF, and UreE, responsible for nickel trafficking and delivering.

F. Musiani, M. Bellucci, S. Ciurli (2011). Model Structures of Helicobacter pylori UreD(H) Domains: A Putative Molecular Recognition Platform. JOURNAL OF CHEMICAL INFORMATION AND MODELING, 51, 1513-1520 [10.1021/ci200183n].

Model Structures of Helicobacter pylori UreD(H) Domains: A Putative Molecular Recognition Platform

MUSIANI, FRANCESCO;BELLUCCI, MATTEO;CIURLI, STEFANO LUCIANO
2011

Abstract

The analysis of the sequence of Helicobacter pylori UreD(H), an accessory protein involved in the activation of urease through the assembly of the Ni2+ - containing active site, revealed the presence of two domains. The structure of these domains was calculated using threading and modeling algorithms. A search for putative binding sites on the protein surface was carried out using dedicated algorithms sensitive to either sequence conservation or structural similarity based on geometry and physicochemical properties. The results suggest that UreD(H) acts as a multifunctional molecular recognition platform facilitating the interaction between apo-urease and the ancillary pro- teins UreG, UreF, and UreE, responsible for nickel trafficking and delivering.
2011
F. Musiani, M. Bellucci, S. Ciurli (2011). Model Structures of Helicobacter pylori UreD(H) Domains: A Putative Molecular Recognition Platform. JOURNAL OF CHEMICAL INFORMATION AND MODELING, 51, 1513-1520 [10.1021/ci200183n].
F. Musiani; M. Bellucci; S. Ciurli
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/107273
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