Cultivation of durum wheat (Triticum turgidum var. durum Desf.) is spread over Continents and durum ranks the 10th most cultivated cereal worldwide. However, Durum wheat can be considered orphan crop as compared to bread wheat. With the aim of reverting the trend, the Durum Wheat Expert Working Group, Wheat Initiative, managed to coordinate International collaborative projects to develop a complete range of genomics and pre-breeding tools. In 2023, a new Platinum-quality reference genome based on PACBIO-HiFi long-read 35X, Bionano optical mapping and Hi-C (Svevo RefSeq2.0) was generated. A complete and accurate gene annotation and transcription atlas was then obtained by RNAseq and ISOseq sequencing 58 diverse tissues/organs and from Svevo grown under optimal conditions, sampled at different developmental stages and exposed to biotic and abiotic stresses. Gene network analysis with the objective of identifying the hubs most relevant for spike and kernel development and response to stress is underway (see Cappucci et al., this conference). The Svevo RefSeq v2.0 paves the way to a comprehensive molecular investigation of genetic diversity in tetraploid wheat germplasm being achieved based on the classical pangenome pyramid model. A Global Durum Genomic Resource including 3.142 tetraploids was genotyped with the iSelect 90K SNP array and the resulting phylogenetic and population structure analysis allowed to select a Tetraploid Core Collection (TCC) of key-genotypes (including wild and domesticated emmer, T. turgidum subspecies, durum wheat landraces and modern varieties) representing the pangenome pyramid, structured into three layers (the gold, silver and bronze, namely). The consortium PACBIO sequenced 40 highly diverse genomes at gold level, and now targets 96 genomes for the silver level and 450 for the bronze level, respectively. Detailed population structure, demography, selective sweeps, haplotype-distribution and transmission rate from ancestral genotypes up to T. turgidum landraces and varieties will be presented. The Global Durum Genomics Resource is currently under distribution and phenotypic evaluation. Characterization has already been started fo: (i) phenology, (ii) resistance to main wheat diseases as yellow rust, leaf rust, stem rust, Septoria tritici blotch, Soil-Borne Cereal Mosaic Virus (SBCMV), fusarium head blight (FHB), (ii) root system architecture, (iii) grain Yield components and grain quality. Herein, we will provide an updated overview on the most recent achievements.
Maccaferri, M., Mazzucotelli, E., Forestan, C., Bozzoli, M., Muhammad Awais, F., Desiderio, F., et al. (2024). THE COLLABORATIVE GENOMICS TOOLS DEVELOPED BY THE DURUM WHEAT COMMUNITY: FROM THE PLATINUM-QUALITY SVEVO REFSEQ V2.0 TO THE GLOBAL DURUM GENOMIC RESOURCE AND THE TETRAPLOID WHEAT PANGENOME PYRAMID.
THE COLLABORATIVE GENOMICS TOOLS DEVELOPED BY THE DURUM WHEAT COMMUNITY: FROM THE PLATINUM-QUALITY SVEVO REFSEQ V2.0 TO THE GLOBAL DURUM GENOMIC RESOURCE AND THE TETRAPLOID WHEAT PANGENOME PYRAMID
MACCAFERRI M.
;FORESTAN C.;BOZZOLI M.;SALVI S.;TUBEROSA R.
2024
Abstract
Cultivation of durum wheat (Triticum turgidum var. durum Desf.) is spread over Continents and durum ranks the 10th most cultivated cereal worldwide. However, Durum wheat can be considered orphan crop as compared to bread wheat. With the aim of reverting the trend, the Durum Wheat Expert Working Group, Wheat Initiative, managed to coordinate International collaborative projects to develop a complete range of genomics and pre-breeding tools. In 2023, a new Platinum-quality reference genome based on PACBIO-HiFi long-read 35X, Bionano optical mapping and Hi-C (Svevo RefSeq2.0) was generated. A complete and accurate gene annotation and transcription atlas was then obtained by RNAseq and ISOseq sequencing 58 diverse tissues/organs and from Svevo grown under optimal conditions, sampled at different developmental stages and exposed to biotic and abiotic stresses. Gene network analysis with the objective of identifying the hubs most relevant for spike and kernel development and response to stress is underway (see Cappucci et al., this conference). The Svevo RefSeq v2.0 paves the way to a comprehensive molecular investigation of genetic diversity in tetraploid wheat germplasm being achieved based on the classical pangenome pyramid model. A Global Durum Genomic Resource including 3.142 tetraploids was genotyped with the iSelect 90K SNP array and the resulting phylogenetic and population structure analysis allowed to select a Tetraploid Core Collection (TCC) of key-genotypes (including wild and domesticated emmer, T. turgidum subspecies, durum wheat landraces and modern varieties) representing the pangenome pyramid, structured into three layers (the gold, silver and bronze, namely). The consortium PACBIO sequenced 40 highly diverse genomes at gold level, and now targets 96 genomes for the silver level and 450 for the bronze level, respectively. Detailed population structure, demography, selective sweeps, haplotype-distribution and transmission rate from ancestral genotypes up to T. turgidum landraces and varieties will be presented. The Global Durum Genomics Resource is currently under distribution and phenotypic evaluation. Characterization has already been started fo: (i) phenology, (ii) resistance to main wheat diseases as yellow rust, leaf rust, stem rust, Septoria tritici blotch, Soil-Borne Cereal Mosaic Virus (SBCMV), fusarium head blight (FHB), (ii) root system architecture, (iii) grain Yield components and grain quality. Herein, we will provide an updated overview on the most recent achievements.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
