The in vitro degradation of a commercial biodegradable hydroxyapatite (HA)-polymer(epsilon-caprolactone)-poly(oxyethylene)-poliepsilon-caprolactone) block copolymer PCL-POE-PCL) composite membrane was investigated by Raman spectroscopy, in two enzymatic solutions, at 37°C: esterase and -chymotrypsin in saline phosphate buffer (SPB, pH 7.4). The degradation was found to be faster in the enzymatic media than in SPB and alkaline solutions. The fastest degradation rate was observed in esterase solution. The trend of properly chosen Raman intensity ratios indicated that both polymeric and apatitic components are involved in degradation. The former underwent preferential degradation of POE blocks, HA is removed by the degradation media faster than the polymer. Raman spectroscopy proved a valid tool for investigating the degradation of the membrane.
Raman study on the biodegradation of a composite periodontal membrane / M. Reggiani; P. Taddei; A. Tinti; C. Fagnano. - STAMPA. - (2004), pp. 80-85. (Intervento presentato al convegno XVIII National Congress GNSR (National Group of Discussion on Raman Spectroscopy and Non Linear Effects) tenutosi a Perugia nel 17-19 settembre 2003).
Raman study on the biodegradation of a composite periodontal membrane
REGGIANI, MATTEO;TADDEI, PAOLA;TINTI, ANNA;FAGNANO, CONCEZIO
2004
Abstract
The in vitro degradation of a commercial biodegradable hydroxyapatite (HA)-polymer(epsilon-caprolactone)-poly(oxyethylene)-poliepsilon-caprolactone) block copolymer PCL-POE-PCL) composite membrane was investigated by Raman spectroscopy, in two enzymatic solutions, at 37°C: esterase and -chymotrypsin in saline phosphate buffer (SPB, pH 7.4). The degradation was found to be faster in the enzymatic media than in SPB and alkaline solutions. The fastest degradation rate was observed in esterase solution. The trend of properly chosen Raman intensity ratios indicated that both polymeric and apatitic components are involved in degradation. The former underwent preferential degradation of POE blocks, HA is removed by the degradation media faster than the polymer. Raman spectroscopy proved a valid tool for investigating the degradation of the membrane.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
