We have investigated the morphology of the sinus node of the human cardiac conduction system. Until today the sinus node (SN) is described as a heterogeneous system composed of 2 types of cells, namely, P or pale and Tor transitional cells which are immersed in the matrix around the sinus nodal artery. T cells are said to share characteristics of P cells and of peripheral working atrial myocardial cells. This study was carried out on autoptic and explanted specimens using histochemical, immunohistochemical, and electron microscopic methods. Our investigations show that SN tissue has a quite different cellular composition, ie, spherical and/or star-shaped cells organized in clusters with long cytoplasmic processes (type P), transitional cells, similar to myocytes but with a reduced number of sarcomeres (type T) and, finally, as yet not described in the literature, fibroblast-like cells with long bitripolar extensions contacting cells. Interestingly, SN is squared by connective and elastic fibers geometrically arranged. Immunohistochemistry shows that the 3 cell types of the SN node express mesenchymal markers revelatory of their embryological origin. Innervation appears to be more complex than previously thought; we identified a system of synaptophysin-positive cholinergic vesicles dependent on the sympathetic system and parasympathetic fibers expressing S100 protein

T. Balbi, C. Ghimenton, G. Pasquinelli, L. Foroni, M. Grillini, G. Pierini (2011). Advancement in the Examination of the Human Cardiac Sinus Node: An Unexpected Architecture and a Novel Cell Type Could Interest the Forensic Science. THE AMERICAN JOURNAL OF FORENSIC MEDICINE AND PATHOLOGY, 32(2), 112-118 [10.1097/PAF.0b013e3181ce9f23].

Advancement in the Examination of the Human Cardiac Sinus Node: An Unexpected Architecture and a Novel Cell Type Could Interest the Forensic Science

BALBI, TIZIANA;PASQUINELLI, GIANANDREA;FORONI, LAURA;PIERINI, GIOVANNI
2011

Abstract

We have investigated the morphology of the sinus node of the human cardiac conduction system. Until today the sinus node (SN) is described as a heterogeneous system composed of 2 types of cells, namely, P or pale and Tor transitional cells which are immersed in the matrix around the sinus nodal artery. T cells are said to share characteristics of P cells and of peripheral working atrial myocardial cells. This study was carried out on autoptic and explanted specimens using histochemical, immunohistochemical, and electron microscopic methods. Our investigations show that SN tissue has a quite different cellular composition, ie, spherical and/or star-shaped cells organized in clusters with long cytoplasmic processes (type P), transitional cells, similar to myocytes but with a reduced number of sarcomeres (type T) and, finally, as yet not described in the literature, fibroblast-like cells with long bitripolar extensions contacting cells. Interestingly, SN is squared by connective and elastic fibers geometrically arranged. Immunohistochemistry shows that the 3 cell types of the SN node express mesenchymal markers revelatory of their embryological origin. Innervation appears to be more complex than previously thought; we identified a system of synaptophysin-positive cholinergic vesicles dependent on the sympathetic system and parasympathetic fibers expressing S100 protein
2011
T. Balbi, C. Ghimenton, G. Pasquinelli, L. Foroni, M. Grillini, G. Pierini (2011). Advancement in the Examination of the Human Cardiac Sinus Node: An Unexpected Architecture and a Novel Cell Type Could Interest the Forensic Science. THE AMERICAN JOURNAL OF FORENSIC MEDICINE AND PATHOLOGY, 32(2), 112-118 [10.1097/PAF.0b013e3181ce9f23].
T. Balbi; C. Ghimenton; G. Pasquinelli; L. Foroni; M. Grillini; G. Pierini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/102585
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