Ejaculates fromfive clinically healthy dromedary camels (Camelus dromedarius)were used to evaluate the effects of different enzymatic treatments (Amylase, Papain, Spermfluid®) on liquefaction and seminal parameters. After collection, ejaculates were divided into 5 aliquots: (1) kept undiluted (control); or diluted 1:1 with: (2) Tris- Citrate-Fructose (TCF), (3) TCF containing Amylase, (4) TCF containing Papain or (5) Spermfluid® containing Bromelain. At 120 min after dilution, each aliquot was evaluated, at 20-min intervals, for viscosity, motility, viability and agglutination. Only the aliquots diluted with TCF containing Papain underwent complete liquefaction. Sperm motility decreased significantly during the observation times, except for the samples diluted with Spermfluid® (P = 0.005). Diluted samples showed different levels of agglutination, with the lowest being observed in the control and the highest in the Papain-treated samples. The viscosity of dromedary camel ejaculates could be effectively reduced by using the proteolytic enzyme Papain.
MONACO, D., Fatnassi, M., PADALINO, B., Hammadi, M., Khorchani, T., LACALANDRA, G.M. (2016). Effect of α-amylase, papain, and spermfluid® treatments on viscosity and semen parameters of dromedary camel ejaculates. RESEARCH IN VETERINARY SCIENCE, 105, 5-9 [10.1016/j.rvsc.2016.01.003].
Effect of α-amylase, papain, and spermfluid® treatments on viscosity and semen parameters of dromedary camel ejaculates
PADALINO, Barbara;
2016
Abstract
Ejaculates fromfive clinically healthy dromedary camels (Camelus dromedarius)were used to evaluate the effects of different enzymatic treatments (Amylase, Papain, Spermfluid®) on liquefaction and seminal parameters. After collection, ejaculates were divided into 5 aliquots: (1) kept undiluted (control); or diluted 1:1 with: (2) Tris- Citrate-Fructose (TCF), (3) TCF containing Amylase, (4) TCF containing Papain or (5) Spermfluid® containing Bromelain. At 120 min after dilution, each aliquot was evaluated, at 20-min intervals, for viscosity, motility, viability and agglutination. Only the aliquots diluted with TCF containing Papain underwent complete liquefaction. Sperm motility decreased significantly during the observation times, except for the samples diluted with Spermfluid® (P = 0.005). Diluted samples showed different levels of agglutination, with the lowest being observed in the control and the highest in the Papain-treated samples. The viscosity of dromedary camel ejaculates could be effectively reduced by using the proteolytic enzyme Papain.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
