Objectives: To grant an in vivo morphological knowledge of Molar Incisor Hypoplasia affected molars supplemented by a new compound (CPP-ACP) containing bio-available calcium-phosphate ions deficient in hypomineralized tooth anomalies. Methods: Replica models for indirect SEM observation were taken before and after treatment. Ethical cusps biopsies from restorative bond molars were cut to confirm the replicas outcome. Polyvinilxyloxane impressions (n=30) developed in polyether and analyzed by SEM at different magnification (from 500X to 5.000X) were taken from permanent molars of children age 7/10 before and after home topical application of a casein-calcium-phosphate mousse. Cusps biopsies (n=10) of untreated elements were taken as control (T0) and after treatment at three different times (n=30) (T1; T2; T3) and analyzed by SEM/EDX at low and high magnification (1000 X - 20.000X). Results: SEM Replica: enamel porosities (T0), with aspects of “crystallized sponge” evolved after three years (T3) in a more homogeneous layer, where surface roughness smoothens. SEM Biopsy: enamel basic structure with prisms and interprismatic zones is present in both porous and unaffected tissue, however prism borders are indistinct and rods are thin and narrow (20.000X) in affected tissue (T0) with an image of “crumbling rods”. After three years of supplementation (T3) morphology and size of rods are closer to healthy enamel. EDX biopsy: comparison of peaks of Ca, P, O, in between T0 and T3 confirm the improvement of values. Conclusions: Supplementation by CPP-ACP in MIH teeth is dramatically efficient by all methods tested.

Supplementation of Molar Incisor Hypoplasia Affected Teeth: SEM/EDX study” / C. Baroni; S. Chersoni; S. Marchionni; and C. Prati. - In: JOURNAL OF DENTAL RESEARCH. - ISSN 0022-0345. - ELETTRONICO. - 89:(2010), p. 4793. (Intervento presentato al convegno IADR/AADR 88th General Session, Barcelona, Spain, 14-17 July 2010, tenutosi a Barcelona, Spain nel 14-17 July 2010).

Supplementation of Molar Incisor Hypoplasia Affected Teeth: SEM/EDX study”

BARONI, CHIARA;CHERSONI, STEFANO;MARCHIONNI, SILVIA;PRATI, CARLO
2010

Abstract

Objectives: To grant an in vivo morphological knowledge of Molar Incisor Hypoplasia affected molars supplemented by a new compound (CPP-ACP) containing bio-available calcium-phosphate ions deficient in hypomineralized tooth anomalies. Methods: Replica models for indirect SEM observation were taken before and after treatment. Ethical cusps biopsies from restorative bond molars were cut to confirm the replicas outcome. Polyvinilxyloxane impressions (n=30) developed in polyether and analyzed by SEM at different magnification (from 500X to 5.000X) were taken from permanent molars of children age 7/10 before and after home topical application of a casein-calcium-phosphate mousse. Cusps biopsies (n=10) of untreated elements were taken as control (T0) and after treatment at three different times (n=30) (T1; T2; T3) and analyzed by SEM/EDX at low and high magnification (1000 X - 20.000X). Results: SEM Replica: enamel porosities (T0), with aspects of “crystallized sponge” evolved after three years (T3) in a more homogeneous layer, where surface roughness smoothens. SEM Biopsy: enamel basic structure with prisms and interprismatic zones is present in both porous and unaffected tissue, however prism borders are indistinct and rods are thin and narrow (20.000X) in affected tissue (T0) with an image of “crumbling rods”. After three years of supplementation (T3) morphology and size of rods are closer to healthy enamel. EDX biopsy: comparison of peaks of Ca, P, O, in between T0 and T3 confirm the improvement of values. Conclusions: Supplementation by CPP-ACP in MIH teeth is dramatically efficient by all methods tested.
2010
4793
Supplementation of Molar Incisor Hypoplasia Affected Teeth: SEM/EDX study” / C. Baroni; S. Chersoni; S. Marchionni; and C. Prati. - In: JOURNAL OF DENTAL RESEARCH. - ISSN 0022-0345. - ELETTRONICO. - 89:(2010), p. 4793. (Intervento presentato al convegno IADR/AADR 88th General Session, Barcelona, Spain, 14-17 July 2010, tenutosi a Barcelona, Spain nel 14-17 July 2010).
C. Baroni; S. Chersoni; S. Marchionni; and C. Prati
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/99444
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