Beet soil-borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV) are members of the genus Benyvirus. BSBMV has been reported only from the United States while BNYVV has a worldwide distribution. Both viruses are vectored by Polymyxa betae, possess similar host ranges and particles morphology. These viruses are not serologically related but have similar genomic organizations. Field isolates possess four RNA species but some BNYVV isolates contain a fifth RNA. RNA-1 and -2 are essential for infection and replication while RNA-3 and -4 play important roles in plant and vector interactions, respectively. In mixed infections, BNYVV reduces BSBMV accumulation in both susceptible and resistant cultivars. BNYVV and BSBMV cross-study, exploiting their similarities and divergences, can improve investigation of molecular interactions between sugar beets and benyviruses. cDNA copies from all BSBMV RNAs and BNYVV (type P) RNA-1 and -2 were successfully synthesized and infectivity of the in vitro transcribed RNAs evaluated through rub-inoculation onto Chenopodium quinoa. Recombination experiments demonstrated that BNYVV RNA-1 and -2 or BSBMV RNA-1/BNYVV RNA- 2 viral machineries are able to replicate and to encapsidate BSBMV RNA-3 and RNA-4 in planta. The capability of BSBMV RNA-4 to promote BNYVV RNA-1, -2 and -3 transmission through the vector P. betae in Beta vulgaris plants, was also assayed. Finally a new form of BSBMV RNA-4 1,733 nts long has been detected and characterised. Two putative ORFs have been identified which encode two proteins of 32 and 13 kDa
Construction and analysis of genomic, full-length clones for the study of the mechanisms involved in the plant-benyvirus interaction / Ratti C.; M. D’Alonzo; M. Nager Grifo; E. Uberegui Bernad; D. Gilmer; C. Rubies Autonell.. - STAMPA. - 91 (4 Supplement):(2009), pp. S4.38-S4.38. (Intervento presentato al convegno XV National Meeting of the Italian Society for Plant Pathology, tenutosi a Locorotondo (Bari) nel September 28th-october 1st 2009).
Construction and analysis of genomic, full-length clones for the study of the mechanisms involved in the plant-benyvirus interaction.
RATTI, CLAUDIO;D'ALONZO, MASSIMILIANO;RUBIES AUTONELL, CONCEPCION
2009
Abstract
Beet soil-borne mosaic virus (BSBMV) and Beet necrotic yellow vein virus (BNYVV) are members of the genus Benyvirus. BSBMV has been reported only from the United States while BNYVV has a worldwide distribution. Both viruses are vectored by Polymyxa betae, possess similar host ranges and particles morphology. These viruses are not serologically related but have similar genomic organizations. Field isolates possess four RNA species but some BNYVV isolates contain a fifth RNA. RNA-1 and -2 are essential for infection and replication while RNA-3 and -4 play important roles in plant and vector interactions, respectively. In mixed infections, BNYVV reduces BSBMV accumulation in both susceptible and resistant cultivars. BNYVV and BSBMV cross-study, exploiting their similarities and divergences, can improve investigation of molecular interactions between sugar beets and benyviruses. cDNA copies from all BSBMV RNAs and BNYVV (type P) RNA-1 and -2 were successfully synthesized and infectivity of the in vitro transcribed RNAs evaluated through rub-inoculation onto Chenopodium quinoa. Recombination experiments demonstrated that BNYVV RNA-1 and -2 or BSBMV RNA-1/BNYVV RNA- 2 viral machineries are able to replicate and to encapsidate BSBMV RNA-3 and RNA-4 in planta. The capability of BSBMV RNA-4 to promote BNYVV RNA-1, -2 and -3 transmission through the vector P. betae in Beta vulgaris plants, was also assayed. Finally a new form of BSBMV RNA-4 1,733 nts long has been detected and characterised. Two putative ORFs have been identified which encode two proteins of 32 and 13 kDaI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
