Inula viscosa L. (Asteraceae) is a well-known perennial medicinal herb that grows wild in the Mediterranean area. Both folk and traditional medicines ascribe several uses to this plant, such as antipyretic, antiseptic, etc. In Spring 2007, almost 40% of I. viscosa plants cultivated at the Herb Garden “Augusto Rinaldi Ceroni “ of Casola Valsenio (Emilia Romagna, Italy) showed chlorotic mosaic on leaves. Symptomatic samples were collected and analysed. No spherical or elongated virus-like particles were observed by transmission electron microscopy in leaf extracts using a leaf dip method. By applying PAS-ELISA technique, Cucumber mosaic virus alone was detected in all symptomatic plants (more than 20) tested, using a polyclonal antiserum (PVAS 30, American Type Culture Collection, Manassas, VA, USA). CMV from I. viscosa leaves was mechanically transmitted to Nicotiana tabacum L. “Samsun”, which developed systemic mosaic symptoms. To confirm the association of CMV with the diseased plants, total RNA was extracted from the same samples (RNeasy Plant Mini Kit; Qiagen, Hilden, Germany) and analyzed by RT-PCR using CMV-specific primers MP+ and MP- (Lin et al., 2004). The expected 842 bp fragment was amplified only from samples of symptomatic tissue while no amplification product was observed when water or healthy plants were used as a template. RT-PCR products were cloned and sequenced. The sequence obtained (GenBank Accession No. EU432181) had 99% identity with CMV-TN (GenBank Accession No. AB176847) which induces tomato necrosis disease, and several other isolates of subgroup II. To our knowledge, this is the first report of CMV infecting I. viscosa and it adds a new host to the list of more than 1,000 species infected by this virus.
INULA VISCOSA NEW HOST OF CUCUMBER MOSAIC VIRUS
BELLARDI, MARIA GRAZIA
2010
Abstract
Inula viscosa L. (Asteraceae) is a well-known perennial medicinal herb that grows wild in the Mediterranean area. Both folk and traditional medicines ascribe several uses to this plant, such as antipyretic, antiseptic, etc. In Spring 2007, almost 40% of I. viscosa plants cultivated at the Herb Garden “Augusto Rinaldi Ceroni “ of Casola Valsenio (Emilia Romagna, Italy) showed chlorotic mosaic on leaves. Symptomatic samples were collected and analysed. No spherical or elongated virus-like particles were observed by transmission electron microscopy in leaf extracts using a leaf dip method. By applying PAS-ELISA technique, Cucumber mosaic virus alone was detected in all symptomatic plants (more than 20) tested, using a polyclonal antiserum (PVAS 30, American Type Culture Collection, Manassas, VA, USA). CMV from I. viscosa leaves was mechanically transmitted to Nicotiana tabacum L. “Samsun”, which developed systemic mosaic symptoms. To confirm the association of CMV with the diseased plants, total RNA was extracted from the same samples (RNeasy Plant Mini Kit; Qiagen, Hilden, Germany) and analyzed by RT-PCR using CMV-specific primers MP+ and MP- (Lin et al., 2004). The expected 842 bp fragment was amplified only from samples of symptomatic tissue while no amplification product was observed when water or healthy plants were used as a template. RT-PCR products were cloned and sequenced. The sequence obtained (GenBank Accession No. EU432181) had 99% identity with CMV-TN (GenBank Accession No. AB176847) which induces tomato necrosis disease, and several other isolates of subgroup II. To our knowledge, this is the first report of CMV infecting I. viscosa and it adds a new host to the list of more than 1,000 species infected by this virus.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
