SDS-PAGE identification of raw and cooked clams of the Veneridae family

Clam species belonging to the family Veneridae (Mollusca: Bivalvia) represent very important marine products, especially in the European countries. The increasing economic interest in marine edible molluscs has strongly enhanced the need for a routine method of unambiguous recognition of species in Fresh and preserved material, mainly intended to prevent mislabelling and commercial frauds. Here a procedure for identification of soft parts is proposed, by means of SDS-PAGE total protein patterning. In fresh material, this technique allowed recognition of four main regions of diagnostic bands, which have been named V1 (111-99 kDa), V2 (44 kDa), V3 (33-30 kDa) and V4 (19-13 kDa). The main diagnostic region appears to be the V3 band series. SDS-PAGE analysis allows the same technique to be used with denatured samples, such as cooked and preserved soft parts, giving reproducible results, very similar to those of fresh material.