Candidatus Phytoplasma prunorum is associated with the quarantine phytoplasma European stone fruit yellows (ESFY)disease that generally induces yellows, tree decline or die-back and vegetative disorders with typical symptoms such as an early bud break and leaf rolling on most of wild and cultivated Prunus species. Ca. Phytoplasma prunorum causes substantial economic loss due to the decline and death of the infected trees; in order to riduce ESFY spreading, a specific, sensitive molecular diagnostic method is needed to early identify and then remove infected plants. A rapid, sensitive, and reliable diagnostic method based on Spot Real-Time Reverse Transcription-PCR Taqman assay has been developed for Ca. P. prunorum detection and relative quantification.

Detection and relative quantification of Candidatus Phytoplasma prunorum by Spot Real time RT-PCR Taqman assay

RATTI, CLAUDIO;RUBIES AUTONELL, CONCEPCION;POGGI POLLINI, CARLO
2010

Abstract

Candidatus Phytoplasma prunorum is associated with the quarantine phytoplasma European stone fruit yellows (ESFY)disease that generally induces yellows, tree decline or die-back and vegetative disorders with typical symptoms such as an early bud break and leaf rolling on most of wild and cultivated Prunus species. Ca. Phytoplasma prunorum causes substantial economic loss due to the decline and death of the infected trees; in order to riduce ESFY spreading, a specific, sensitive molecular diagnostic method is needed to early identify and then remove infected plants. A rapid, sensitive, and reliable diagnostic method based on Spot Real-Time Reverse Transcription-PCR Taqman assay has been developed for Ca. P. prunorum detection and relative quantification.
Petria- Proceedings of the 13th congress of the mediterranean phytopathological union (MPU)
219
220
Minguzzi S.; Ratti C.; Lanzoni C.; Rubies Autonell C.; Reggiani N.; Poggi Pollini C.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/97542
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