Candidatus Phytoplasma prunorum is associated with the quarantine phytoplasma European stone fruit yellows (ESFY)disease that generally induces yellows, tree decline or die-back and vegetative disorders with typical symptoms such as an early bud break and leaf rolling on most of wild and cultivated Prunus species. Ca. Phytoplasma prunorum causes substantial economic loss due to the decline and death of the infected trees; in order to riduce ESFY spreading, a specific, sensitive molecular diagnostic method is needed to early identify and then remove infected plants. A rapid, sensitive, and reliable diagnostic method based on Spot Real-Time Reverse Transcription-PCR Taqman assay has been developed for Ca. P. prunorum detection and relative quantification.
Detection and relative quantification of Candidatus Phytoplasma prunorum by Spot Real time RT-PCR Taqman assay / Minguzzi S.; Ratti C.; Lanzoni C.; Rubies Autonell C.; Reggiani N.; Poggi Pollini C.. - STAMPA. - 20 (2):(2010), pp. 219-220. (Intervento presentato al convegno 13th congress of the mediterranean phytopathological union (MPU) tenutosi a Roma nel 20-25 June 2010).
Detection and relative quantification of Candidatus Phytoplasma prunorum by Spot Real time RT-PCR Taqman assay
RATTI, CLAUDIO;RUBIES AUTONELL, CONCEPCION;POGGI POLLINI, CARLO
2010
Abstract
Candidatus Phytoplasma prunorum is associated with the quarantine phytoplasma European stone fruit yellows (ESFY)disease that generally induces yellows, tree decline or die-back and vegetative disorders with typical symptoms such as an early bud break and leaf rolling on most of wild and cultivated Prunus species. Ca. Phytoplasma prunorum causes substantial economic loss due to the decline and death of the infected trees; in order to riduce ESFY spreading, a specific, sensitive molecular diagnostic method is needed to early identify and then remove infected plants. A rapid, sensitive, and reliable diagnostic method based on Spot Real-Time Reverse Transcription-PCR Taqman assay has been developed for Ca. P. prunorum detection and relative quantification.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.