Quasispecies composition and tissue distribution of feline coronaviruses (FCoVs) were studied in naturally infected cats. The genomic complexity of FCoVs was investigated using single-strand conformational polymorphism (SSCP) analysis of N and ORF7b amplicons, and the evolutionary process was investigated by sequence-based phylogenetic analysis. SSCP analysis showed high heterogeneity of the FCoV genome which was correlated with the seriousness of the clinical form. The two genomic regions analysed showed different levels of variation; the N region demonstrated significant heterogeneity as compared to ORF7b. Phylogenetic analysis of the nucleotide sequences showed the clear separation of sequences analysed on the basis of virulence and geographical origin. A maximum likelihood analysis of N and ORF7b data sets showed a situation of strong heterogeneity for the N region. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Battilani M., Coradin T., Scagliarini A., Ciulli S., Ostanello F., Prosperi S., et al. (2003). Quasispecies composition and phylogenetic analysis of feline coronaviruses (FCoVs) in naturally infected cats. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 39(2), 141-147 [10.1016/S0928-8244(03)00237-2].
Quasispecies composition and phylogenetic analysis of feline coronaviruses (FCoVs) in naturally infected cats
Battilani M.;Scagliarini A.;Ciulli S.;Ostanello F.;Prosperi S.;Morganti L.
2003
Abstract
Quasispecies composition and tissue distribution of feline coronaviruses (FCoVs) were studied in naturally infected cats. The genomic complexity of FCoVs was investigated using single-strand conformational polymorphism (SSCP) analysis of N and ORF7b amplicons, and the evolutionary process was investigated by sequence-based phylogenetic analysis. SSCP analysis showed high heterogeneity of the FCoV genome which was correlated with the seriousness of the clinical form. The two genomic regions analysed showed different levels of variation; the N region demonstrated significant heterogeneity as compared to ORF7b. Phylogenetic analysis of the nucleotide sequences showed the clear separation of sequences analysed on the basis of virulence and geographical origin. A maximum likelihood analysis of N and ORF7b data sets showed a situation of strong heterogeneity for the N region. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
