This study introduces for the first time a reliable whole blood microsampling method for forensic analysis of cocaine and its metabolites using quantitative dried blood spot (qDBS) technology and UHPLC-MS analysis. This methodology offers accurate and less invasive sampling and aligns with the current trend towards sustainable and accessible analytical methods. Microsampling is subject-friendly, improves logistics, stability and efficiency, marking a shift towards modern forensic practices with wide-ranging application potential. The qDBS-UHPLC-MS method underwent comprehensive validation, confirming its linearity, sensitivity, precision, extraction efficiency and stability. UHPLC-MS achieved effective chromatographic separation and suitable sensitivity, with detection limits between 1.0 and 2.5 ng/mL, and quantitation limits from 2.5 to 7.5 ng/mL. Analytes showed over 85.1 % extraction yield and less than 7.5 % relative standard deviation in precision. Stability tests indicated superior analyte preservation in qDBS at room temperature versus refrigerated plasma, while minimal matrix effect highlighted the sample clean-up efficiency. Application to real samples yielded consistent quali-quantitative results between qDBS and plasma samples, confirming method suitability for forensic cocaine bioanalysis, thus addressing critical needs in drug testing and pharmaco-toxicological analysis.

Palano S., Turoňova D., Protti M., Kujovská Krčmová L., Sardella R., Mladěnka P., et al. (2024). Next generation microsampling towards sustainable forensic analysis: Volumetric DBS for cocaine and metabolites. MICROCHEMICAL JOURNAL, 203, 1-7 [10.1016/j.microc.2024.110937].

Next generation microsampling towards sustainable forensic analysis: Volumetric DBS for cocaine and metabolites

Protti M.
Co-primo
;
Mandrioli R.;Mercolini L.
Ultimo
2024

Abstract

This study introduces for the first time a reliable whole blood microsampling method for forensic analysis of cocaine and its metabolites using quantitative dried blood spot (qDBS) technology and UHPLC-MS analysis. This methodology offers accurate and less invasive sampling and aligns with the current trend towards sustainable and accessible analytical methods. Microsampling is subject-friendly, improves logistics, stability and efficiency, marking a shift towards modern forensic practices with wide-ranging application potential. The qDBS-UHPLC-MS method underwent comprehensive validation, confirming its linearity, sensitivity, precision, extraction efficiency and stability. UHPLC-MS achieved effective chromatographic separation and suitable sensitivity, with detection limits between 1.0 and 2.5 ng/mL, and quantitation limits from 2.5 to 7.5 ng/mL. Analytes showed over 85.1 % extraction yield and less than 7.5 % relative standard deviation in precision. Stability tests indicated superior analyte preservation in qDBS at room temperature versus refrigerated plasma, while minimal matrix effect highlighted the sample clean-up efficiency. Application to real samples yielded consistent quali-quantitative results between qDBS and plasma samples, confirming method suitability for forensic cocaine bioanalysis, thus addressing critical needs in drug testing and pharmaco-toxicological analysis.
2024
Palano S., Turoňova D., Protti M., Kujovská Krčmová L., Sardella R., Mladěnka P., et al. (2024). Next generation microsampling towards sustainable forensic analysis: Volumetric DBS for cocaine and metabolites. MICROCHEMICAL JOURNAL, 203, 1-7 [10.1016/j.microc.2024.110937].
Palano S.; Turoňova D.; Protti M.; Kujovská Krčmová L.; Sardella R.; Mladěnka P.; Mandrioli R.; Girotti S.; Mercolini L....espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/972015
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