Background: 'Men having sex with men' (MSM) represent a key population with a significant prevalence of pharyngeal Neisseria gonorrhoeae (NG) infections and a high rate of antimicrobial resistance genes in the pharyngeal microbiome. As NG can acquire antibiotic resistance genes from other commensal oropharyngeal bacteria, monitoring the prevalence of these resistance determinants is critical to curtail the spread of NG-resistant strains. Purpose and research design: Here, we assessed the distribution of five resistance genes (pen (A), mtr (R), gyr (A), par (C), msr (D)) in the oropharynx of 164 MSM, attending an Outpatient clinic for STI screening. Results: The most frequently detected resistance gene was msr (D) (88.4%), followed by gyr (A) (67.1%). The distribution of resistance genes was not influenced by pharyngeal gonorrhea nor by the HIV status, whereas a younger age was associated with mtr (R) presence (p = .008). Subjects using mouthwash exhibited significantly lower levels of mtr (R) (p = .0005). Smoking habit was associated with a higher prevalence of par (C) (p = .02). A noteworthy association was observed between the presence of msr (D) gene and the use of antibiotics (p = .014). Conclusions: Our findings reveal an enrichment of antimicrobial resistance genes in the oropharynx of MSM. These insights could aid in the development of screening programs and antimicrobial stewardship initiatives targeting populations at heightened risk of pharyngeal gonorrhea.

Antimicrobial resistance determinants in the oropharyngeal microbiome of 'men having sex with men' attending an sexually transmitted infection clinic / Djusse, Marielle Ezekielle; Gaspari, Valeria; Morselli, Sara; Rapparini, Luca; Foschi, Claudio; Ambretti, Simone; Lazzarotto, Tiziana; Piraccini, Bianca Maria; Marangoni, Antonella. - In: INTERNATIONAL JOURNAL OF STD & AIDS. - ISSN 0956-4624. - ELETTRONICO. - N/A:(2024), pp. 1-5. [10.1177/09564624241255163]

Antimicrobial resistance determinants in the oropharyngeal microbiome of 'men having sex with men' attending an sexually transmitted infection clinic

Djusse, Marielle Ezekielle;Morselli, Sara;Rapparini, Luca;Foschi, Claudio
;
Ambretti, Simone;Lazzarotto, Tiziana;Piraccini, Bianca Maria;Marangoni, Antonella
2024

Abstract

Background: 'Men having sex with men' (MSM) represent a key population with a significant prevalence of pharyngeal Neisseria gonorrhoeae (NG) infections and a high rate of antimicrobial resistance genes in the pharyngeal microbiome. As NG can acquire antibiotic resistance genes from other commensal oropharyngeal bacteria, monitoring the prevalence of these resistance determinants is critical to curtail the spread of NG-resistant strains. Purpose and research design: Here, we assessed the distribution of five resistance genes (pen (A), mtr (R), gyr (A), par (C), msr (D)) in the oropharynx of 164 MSM, attending an Outpatient clinic for STI screening. Results: The most frequently detected resistance gene was msr (D) (88.4%), followed by gyr (A) (67.1%). The distribution of resistance genes was not influenced by pharyngeal gonorrhea nor by the HIV status, whereas a younger age was associated with mtr (R) presence (p = .008). Subjects using mouthwash exhibited significantly lower levels of mtr (R) (p = .0005). Smoking habit was associated with a higher prevalence of par (C) (p = .02). A noteworthy association was observed between the presence of msr (D) gene and the use of antibiotics (p = .014). Conclusions: Our findings reveal an enrichment of antimicrobial resistance genes in the oropharynx of MSM. These insights could aid in the development of screening programs and antimicrobial stewardship initiatives targeting populations at heightened risk of pharyngeal gonorrhea.
2024
Antimicrobial resistance determinants in the oropharyngeal microbiome of 'men having sex with men' attending an sexually transmitted infection clinic / Djusse, Marielle Ezekielle; Gaspari, Valeria; Morselli, Sara; Rapparini, Luca; Foschi, Claudio; Ambretti, Simone; Lazzarotto, Tiziana; Piraccini, Bianca Maria; Marangoni, Antonella. - In: INTERNATIONAL JOURNAL OF STD & AIDS. - ISSN 0956-4624. - ELETTRONICO. - N/A:(2024), pp. 1-5. [10.1177/09564624241255163]
Djusse, Marielle Ezekielle; Gaspari, Valeria; Morselli, Sara; Rapparini, Luca; Foschi, Claudio; Ambretti, Simone; Lazzarotto, Tiziana; Piraccini, Bianca Maria; Marangoni, Antonella
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/969934
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