The single-channel behavior of the hyperpolarization-activated, ClC-2- like inwardly rectifying Cl- current (I(Clh)), induced by long-term dibutyryl-cyclic-AMP-treated cultured cortical rat astrocytes, was analyzed with the patch-clamp technique. In outside-out patches in symmetrical 144 mM Cl- solutions, openings of hyperpolarization-activated small-conductance Cl- channels revealed burst activity of two equidistant conductance levels of 3 and 6 pS. The unitary openings displayed slow activation kinetics. The probabilities of the closed and conducting states were consistent with a double-barrelled structure of the channel protein. These results suggest that the astrocytic ClC-2-like Cl- current I(Clh) is mediated by a small- conductance Cl- channel, which has the same structural motif as the Cl- channel prototype ClC-0. (C) 2000 Federation of European Biochemical Societies.
Single-channel analysis of a ClC-2-like chloride conductance in cultured rat cortical astrocytes / Nobile M.; Pusch M.; Rapisarda C.; Ferroni S.. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 479:1-2(2000), pp. 10-14. [10.1016/S0014-5793(00)01876-7]
Single-channel analysis of a ClC-2-like chloride conductance in cultured rat cortical astrocytes
Rapisarda C.;Ferroni S.
2000
Abstract
The single-channel behavior of the hyperpolarization-activated, ClC-2- like inwardly rectifying Cl- current (I(Clh)), induced by long-term dibutyryl-cyclic-AMP-treated cultured cortical rat astrocytes, was analyzed with the patch-clamp technique. In outside-out patches in symmetrical 144 mM Cl- solutions, openings of hyperpolarization-activated small-conductance Cl- channels revealed burst activity of two equidistant conductance levels of 3 and 6 pS. The unitary openings displayed slow activation kinetics. The probabilities of the closed and conducting states were consistent with a double-barrelled structure of the channel protein. These results suggest that the astrocytic ClC-2-like Cl- current I(Clh) is mediated by a small- conductance Cl- channel, which has the same structural motif as the Cl- channel prototype ClC-0. (C) 2000 Federation of European Biochemical Societies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
