The invention relates to a subtilisin BPN' variant or homologue thereof, comprising new mutations compared to subtilisin BPN' represented by SEQ ID NO: 2 or a homologue sequence thereof. Such mutations may occur at amino acid positions selected from the group consisting of L96, D99, A223 and S224. The invention further relates to a method for enzymatically synthesizing a peptide by coupling peptide fragments, wherein the coupling is catalyzed by said subtilisin BPN' variant or homologue thereof.
Toplak, A. (2021). SUBTILISIN VARIANTS AND THEIR USE.
SUBTILISIN VARIANTS AND THEIR USE
Cabri W.;
2021
Abstract
The invention relates to a subtilisin BPN' variant or homologue thereof, comprising new mutations compared to subtilisin BPN' represented by SEQ ID NO: 2 or a homologue sequence thereof. Such mutations may occur at amino acid positions selected from the group consisting of L96, D99, A223 and S224. The invention further relates to a method for enzymatically synthesizing a peptide by coupling peptide fragments, wherein the coupling is catalyzed by said subtilisin BPN' variant or homologue thereof.File in questo prodotto:
Eventuali allegati, non sono esposti
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
