The invention relates to a subtilisin BPN' variant or homologue thereof, comprising new mutations compared to subtilisin BPN' represented by SEQ ID NO: 2 or a homologue sequence thereof. Such mutations may occur at amino acid positions selected from the group consisting of L96, D99, A223 and S224. The invention further relates to a method for enzymatically synthesizing a peptide by coupling peptide fragments, wherein the coupling is catalyzed by said subtilisin BPN' variant or homologue thereof.
SUBTILISIN VARIANTS AND THEIR USE
Cabri W.;
2021
Abstract
The invention relates to a subtilisin BPN' variant or homologue thereof, comprising new mutations compared to subtilisin BPN' represented by SEQ ID NO: 2 or a homologue sequence thereof. Such mutations may occur at amino acid positions selected from the group consisting of L96, D99, A223 and S224. The invention further relates to a method for enzymatically synthesizing a peptide by coupling peptide fragments, wherein the coupling is catalyzed by said subtilisin BPN' variant or homologue thereof.File in questo prodotto:
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