The hemopoietin stem cell factor (SCF) and its receptor c-kit are expressed in some tumoral cells, including neuroblastoma (NB) cells. We have investigated the effect of retinoic acid (RA), one of the most active differentiating agents on human NB cells, on the SCF production by human neuroblastoma cell lines. SCF concentration was determined by immunoenzymatic assay in the supernatants of seven neuroblastoma cell lines. All cell lines except one showed detectable amounts of SCF in the supernatant in basal culture conditions. A progressive increase pattern of the SCF concentration over time, was common to all SCF secreting cell lines, both unstimulated and RA-stimulated. Moreover, after 48 and 72 hours-exposure to RA, SCF concentrations were higher than in the untreated controls (p<0.01). Membrane SCF mRNA isoform was also detected by reverse-transcription polymerase chain reaction. These effects demonstrated that RA, besides inducing neuronal differentiation, enhanced SCF production in neuroblastoma cell lines.

Retinoic acid modulates stem cell factor secretion by human neuroblastoma cell lines / Strippoli P.; Bagnara G.P.; Montanaro L.; Timeus F.; Ferreri A.M.; Rocchi P.. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - STAMPA. - 20:6 B(2000), pp. 4361-4366.

Retinoic acid modulates stem cell factor secretion by human neuroblastoma cell lines

Strippoli P.;Bagnara G. P.;Montanaro L.;Ferreri A. M.;Rocchi P.
2000

Abstract

The hemopoietin stem cell factor (SCF) and its receptor c-kit are expressed in some tumoral cells, including neuroblastoma (NB) cells. We have investigated the effect of retinoic acid (RA), one of the most active differentiating agents on human NB cells, on the SCF production by human neuroblastoma cell lines. SCF concentration was determined by immunoenzymatic assay in the supernatants of seven neuroblastoma cell lines. All cell lines except one showed detectable amounts of SCF in the supernatant in basal culture conditions. A progressive increase pattern of the SCF concentration over time, was common to all SCF secreting cell lines, both unstimulated and RA-stimulated. Moreover, after 48 and 72 hours-exposure to RA, SCF concentrations were higher than in the untreated controls (p<0.01). Membrane SCF mRNA isoform was also detected by reverse-transcription polymerase chain reaction. These effects demonstrated that RA, besides inducing neuronal differentiation, enhanced SCF production in neuroblastoma cell lines.
2000
Retinoic acid modulates stem cell factor secretion by human neuroblastoma cell lines / Strippoli P.; Bagnara G.P.; Montanaro L.; Timeus F.; Ferreri A.M.; Rocchi P.. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - STAMPA. - 20:6 B(2000), pp. 4361-4366.
Strippoli P.; Bagnara G.P.; Montanaro L.; Timeus F.; Ferreri A.M.; Rocchi P.
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/961903
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? ND
social impact