Background. During haemodialysis blood-membrane contact causes the release of the content of platelet α-granules, which contain platelet-derived growth factor (PDGF). In view of its possible role in accelerated atherosclerotic processes, we evaluated the intra- and post-dialytic changes in PDGF-AB serum levels during haemodialysis sessions performed using a cellulosic membrane. Methods. Using the ELISA method, PDGF-AB, platelet factor-4 (PF4) and β-thromboglobulin (β-TG) levels were determined in peripheral blood, as well as in arterial and venous haemodialyser lines, in 10 patients each of whom underwent five consecutive dialysis sessions with a CU membrane. Blood samples were taken at 0, 15, 30, 60, 120, 180 and 240 min during dialysis and at 1, 4 and 20 h after the end of the session. In the same group of patients the levels of the same molecules were also determined after a heparin bolus injection of 4500 IU, blood samples were taken at 0, 15 and 30 min after injection of the bolus. Results. PDGF-AB serum levels increased, remained consistently high during the haemodialysis session (in particular +134 ± 20% after 30 min, P < 0.001, and +140 ± 5% after 240 min, P < 0.001) and returned to basal values only after 20 h following the end of the session. PF4 and β-TG showed a similar trend to PDGF. The heparin bolus injection caused only a small increase (+15 ± 5% at 30 min) in PDGF-AB serum levels. Conclusions. PDGF-AB is released during dialysis mainly as consequence of the blood-membrane contact and it returns only slowly to basal values.

Cianciolo G., Stefoni S., Zanchelli F., Iannelli S., Coli L., Borgino L.C., et al. (1999). PDGF-AB release during and after haemodialysis procedure. NEPHROLOGY DIALYSIS TRANSPLANTATION, 14(10), 2413-2419 [10.1093/ndt/14.10.2413].

PDGF-AB release during and after haemodialysis procedure

Stefoni S.;De Sanctis L. B.;Stefoni V.;La Manna G.
1999

Abstract

Background. During haemodialysis blood-membrane contact causes the release of the content of platelet α-granules, which contain platelet-derived growth factor (PDGF). In view of its possible role in accelerated atherosclerotic processes, we evaluated the intra- and post-dialytic changes in PDGF-AB serum levels during haemodialysis sessions performed using a cellulosic membrane. Methods. Using the ELISA method, PDGF-AB, platelet factor-4 (PF4) and β-thromboglobulin (β-TG) levels were determined in peripheral blood, as well as in arterial and venous haemodialyser lines, in 10 patients each of whom underwent five consecutive dialysis sessions with a CU membrane. Blood samples were taken at 0, 15, 30, 60, 120, 180 and 240 min during dialysis and at 1, 4 and 20 h after the end of the session. In the same group of patients the levels of the same molecules were also determined after a heparin bolus injection of 4500 IU, blood samples were taken at 0, 15 and 30 min after injection of the bolus. Results. PDGF-AB serum levels increased, remained consistently high during the haemodialysis session (in particular +134 ± 20% after 30 min, P < 0.001, and +140 ± 5% after 240 min, P < 0.001) and returned to basal values only after 20 h following the end of the session. PF4 and β-TG showed a similar trend to PDGF. The heparin bolus injection caused only a small increase (+15 ± 5% at 30 min) in PDGF-AB serum levels. Conclusions. PDGF-AB is released during dialysis mainly as consequence of the blood-membrane contact and it returns only slowly to basal values.
1999
Cianciolo G., Stefoni S., Zanchelli F., Iannelli S., Coli L., Borgino L.C., et al. (1999). PDGF-AB release during and after haemodialysis procedure. NEPHROLOGY DIALYSIS TRANSPLANTATION, 14(10), 2413-2419 [10.1093/ndt/14.10.2413].
Cianciolo G.; Stefoni S.; Zanchelli F.; Iannelli S.; Coli L.; Borgino L.C.; De Sanctis L.B.; Stefoni V.; De Pascalis A.; Isola E.; La Manna G....espandi
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/960194
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 21
  • ???jsp.display-item.citation.isi??? ND
social impact