Circular dichroism (CD) spectroscopy can be successfully used to study the assembly of homoguanylic and guanosine-rich oligonucleotides. The CD corresponding to the low-energy bands of the guanine spectrum in the 250-290 nm region is mainly determined by exciton interaction between the guanine chromophores; it gives information on the relative skew of G-quartets and on their distortion from the classical structure found in the four-stranded helix of poly(G). The handedness of the sugar-phosphate backbone can be deduced indirectly only if the conformation of the bases around the glycosyl bond is known.
Gottarelli G., Masiero S., Spada G.P. (1998). The use of CD spectroscopy for the study of the self-assembly of guanine derivatives. ENANTIOMER, 3(6), 429-438.
The use of CD spectroscopy for the study of the self-assembly of guanine derivatives
Gottarelli G.
;Masiero S.;Spada G. P.
1998
Abstract
Circular dichroism (CD) spectroscopy can be successfully used to study the assembly of homoguanylic and guanosine-rich oligonucleotides. The CD corresponding to the low-energy bands of the guanine spectrum in the 250-290 nm region is mainly determined by exciton interaction between the guanine chromophores; it gives information on the relative skew of G-quartets and on their distortion from the classical structure found in the four-stranded helix of poly(G). The handedness of the sugar-phosphate backbone can be deduced indirectly only if the conformation of the bases around the glycosyl bond is known.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
