Background: We evaluated the prognostic role of 13-gene DNA methylation analysis by oral brushing repeatedly performed during the follow-up of patients surgically treated for oral cancer.Methods: This is a nested case-control study including 61 patients for a total of 64 outcomes (2/61 patients experienced multiple relapses). Samples were collected at baseline (4-10 months after OSCC resection) and repeatedly every 4-10 months until relapse or death. DNA methylation scores were classified as persistently positive, persistently negative, or mixed.Results: Twenty cases who had persistently positive scores and 30 cases with mixed scores had, respectively, an almost 42-fold (p < 0.001) and 32-fold (p = 0.006) higher likelihood of relapse, compared to 14 patients with persistently negative scores. The last score before reoccurrence was positive in 18/19 secondary events.Conclusions: The 13-gene DNA methylation analysis may be considered for the surveillance of patients treated for oral carcinoma.
Thirteen-gene DNA methylation analysis of oral brushing samples: A potential surveillance tool for periodic monitoring of treated patients with oral cancer / Gissi, Davide B; Rossi, Roberto; Lenzi, Jacopo; Tarsitano, Achille; Gabusi, Andrea; Balbi, Tiziana; Montebugnoli, Lucio; Marchetti, Claudio; Foschini, Maria Pia; Morandi, Luca. - In: HEAD & NECK. - ISSN 1097-0347. - ELETTRONICO. - Online ahead of print:(2024), pp. 1-12. [10.1002/hed.27621]
Thirteen-gene DNA methylation analysis of oral brushing samples: A potential surveillance tool for periodic monitoring of treated patients with oral cancer
Gissi, Davide B;Rossi, Roberto;Lenzi, Jacopo;Tarsitano, Achille;Gabusi, Andrea;Montebugnoli, Lucio;Marchetti, Claudio;Foschini, Maria Pia;Morandi, Luca
2024
Abstract
Background: We evaluated the prognostic role of 13-gene DNA methylation analysis by oral brushing repeatedly performed during the follow-up of patients surgically treated for oral cancer.Methods: This is a nested case-control study including 61 patients for a total of 64 outcomes (2/61 patients experienced multiple relapses). Samples were collected at baseline (4-10 months after OSCC resection) and repeatedly every 4-10 months until relapse or death. DNA methylation scores were classified as persistently positive, persistently negative, or mixed.Results: Twenty cases who had persistently positive scores and 30 cases with mixed scores had, respectively, an almost 42-fold (p < 0.001) and 32-fold (p = 0.006) higher likelihood of relapse, compared to 14 patients with persistently negative scores. The last score before reoccurrence was positive in 18/19 secondary events.Conclusions: The 13-gene DNA methylation analysis may be considered for the surveillance of patients treated for oral carcinoma.File | Dimensione | Formato | |
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